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The Study of ATRA-Induced Differentiation of the HL-60 Cell Line

ABSTRACT: Shotgun mass-spectrometric experiment was performed with HL-60 cells induced to differentiation at 0, 3, 24, 48 and 96h after ATRA treatment. Obtained data has been used for label-free quantification by SPIRE platform based on spectral count. Results on proteome profiling were used for up-stream regulator search in geneXplain platform (http://genexplain.com/).

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Cell Suspension Culture

DISEASE(S): Acute Leukemia

SUBMITTER: Svetlana Novikova

LAB HEAD: Victor Zgoda

PROVIDER: PXD006768 | Pride | 2021-09-07

REPOSITORIES: Pride

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Omics Technologies to Decipher Regulatory Networks in Granulocytic Cell Differentiation.

Novikova Svetlana S Tikhonova Olga O Kurbatov Leonid L Farafonova Tatiana T Vakhrushev Igor I Lupatov Alexey A Yarygin Konstantin K Zgoda Victor V

Biomolecules 20210618 6

Induced granulocytic differentiation of human leukemic cells under all-<i>trans</i>-retinoid acid (ATRA) treatment underlies differentiation therapy of acute myeloid leukemia. Knowing the regulation of this process it is possible to identify potential targets for antileukemic drugs and develop novel approaches to differentiation therapy. In this study, we have performed transcriptomic and proteomic profiling to reveal up- and down-regulated transcripts and proteins during time-course experiments ...[more]

PMID: 34207065

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