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ISDoT: In Situ Decellularization of Tissues for proteomic analysis of native extracellular matrix

ABSTRACT: The extracellular matrix (ECM) is a master regulator of cellular phenotype and behavior. It plays a critical role in both normal tissue homeostasis and disease pathology. We have developed a fast and efficient approach to enhance the study of ECM composition and structure. Termed In Situ Decellularization of Tissues (ISDoT), it allows whole organs to be decellularized leaving native ECM architecture intact. We demonstrate how our approach enriches for ECM components allowing deep quantitative proteomic interrogation of the ECM. Using the ISDoT procedure, native ECM under normal and pathological conditions can be catalogued in unprecedented detail.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lung, Lymph Node, Mammary Gland

DISEASE(S): Oral Cavity Cancer,Breast Cancer

SUBMITTER: Thomas Cox

LAB HEAD: Janine Erler

PROVIDER: PXD006579 | Pride | 2017-06-12

REPOSITORIES: Pride

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ISDoT: in situ decellularization of tissues for high-resolution imaging and proteomic analysis of native extracellular matrix.

Mayorca-Guiliani Alejandro E AE Madsen Chris D CD Cox Thomas R TR Horton Edward R ER Venning Freja A FA Erler Janine T JT

Nature medicine 20170612 7

The extracellular matrix (ECM) is a master regulator of cellular phenotype and behavior. It has a crucial role in both normal tissue homeostasis and disease pathology. Here we present a fast and efficient approach to enhance the study of ECM composition and structure. Termed in situ decellularization of tissues (ISDoT), it allows whole organs to be decellularized, leaving native ECM architecture intact. These three-dimensional decellularized tissues can be studied using high-resolution fluoresce ...[more]

PMID: 28604702

Publication: 1/2

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