Quantitative proteomics after spinal cord injury in a regenerative and a non-regenerative stage in Xenopus laevis
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ABSTRACT: The capacity to regenerate the spinal cord after an injury is a coveted trait that only a limited group of non-mammalian organisms can achieve. In Xenopus laevis, this capacity is only present during larval or tadpole stages, but is absent during postmetamorphic frog stages. This provides an excellent model for comparative studies between a regenerative and a non-regenerative stage to identify the cellular and molecular mechanisms that explain the difference in regenerative potential. Here, we used iTRAQ chemistry to obtain a quantitative proteome of the spinal cord 1 day after a transection injury, and used sham operated animals as controls. We quantified a total of 6,384 proteins, with 172 showing significant differential expression in the regenerative stage and 240 in the non-regenerative stage, with an overlap of only 14 proteins. Functional enrichment analysis revealed that while the regenerative stage downregulated synapse/vesicle and mitochondrial proteins, the non-regenerative stage upregulated lipid metabolism proteins, and downregulated ribosomal and translation control proteins. Furthermore, STRING network analysis showed that proteins belonging to these groups are highly interconnected, providing interesting candidates for future functional studies.
INSTRUMENT(S): Q Exactive
ORGANISM(S): Xenopus Laevis (african Clawed Frog)
TISSUE(S): Nerve Cord
SUBMITTER: Liangliang Sun
LAB HEAD: Liangliang Sun
PROVIDER: PXD006993 | Pride | 2018-01-25
REPOSITORIES: Pride
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