Proteomics

Dataset Information

34

Rat colon LC-MSMS


ABSTRACT: Dietary fats have been shown to affect gut microbiota composition and aging gene transcription of middle-aged rats at a normal dose, but little is known about such an effect on gut barrier. In colon, the main component of mucus layer is Muc2, produced by the goblet cells. This study investigated the changes in Muc2 expression, goblet cells proliferation, TLRs and inflammatory cytokines in the colon of middle-aged rats. Proteome technology was applied to explore the possible molecular mechanisms. The results indicated that intake of fish oil at a normal dose downregulated colonic Muc2 expression, and this negative effect of fish oil probably involved the suppression of mucin glycosylation process.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Rattus norvegicus  

TISSUE(S): Colon

DISEASE(S): Not Available

SUBMITTER: Yafang Ma  

LAB HEAD: Chunbao Li

PROVIDER: PXD007229 | Pride | 2018-01-02

REPOSITORIES: Pride

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Publications

Intake of Fish Oil Specifically Modulates Colonic Muc2 Expression in Middle-Aged Rats by Suppressing the Glycosylation Process.

Ma Yafang Y   Zhou Guanghong G   Li Yingqiu Y   Zhu Yingying Y   Yu Xiaobo X   Zhao Fan F   Li He H   Xu Xinglian X   Li Chunbao C  

Molecular nutrition & food research 20180129 4


SCOPE:Dietary fats have been shown to affect gut microbiota composition and aging gene expression of middle-aged rats at a normal dose, but little is known about such an effect on gut barrier. In this study, the changes in colonic Muc2 expression are investigated and the underlying mechanism is also proposed. METHODS AND RESULTS:36 middle-aged Sprague-Dawley rats are assigned to one of the diets containing soybean oil, lard, or fish oil (4%). The rats are fed for 5 weeks and then goblet cells, M  ...[more]

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