Protein profile at different cellular fractions of Mycobacterium tuberculosis strains after exposure to Isoniazid
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ABSTRACT: Out of the 10 million of tuberculosis (TB) cases estimated in the world, around 14% are isoniazid (INH) resistant among new cases and 29% among previously treated cases in the last decade. INH is one of the oldest but also one of the more potent drugs to eliminate Mycobacterium tuberculosis (Mtb), the causing agent of TB. Because of the efficiency of isoniazid (INH) against Mycobacterium tuberculosis (Mtb), many studies are still focused in better understand its role in different bacterial metabolic pathways. We recently conducted a study that evaluated the changes in the protein abundance at different cellular fractions when clonal strains of Mtb developed INH resistance in the clinical and laboratory setting. Here, we want to establish which of the protein changes occurred or started because of the initial exposure to INH. Additionally, we wanted to evaluate if those changes happen differently in strains that are sensitive or resistant to INH, evaluating different cellular compartments from two different genetic lineages of Mtb. For this purpose, we analyzed the proteome of each cellular compartment (cytosol, cell wall, membrane and secreted proteins) through liquid chromatography (nano-HPLC) coupled to mass spectrometry using the Orbitrap Velos instrument and a t-test to perform the statistical analysis for each pair comparison.
INSTRUMENT(S): LTQ Orbitrap Velos
ORGANISM(S): Mycobacterium Tuberculosis H37rv
TISSUE(S): Prokaryotic Cell
DISEASE(S): Pulmonary Tuberculosis
SUBMITTER: Luisa Nieto Ramirez
LAB HEAD: Karen M. Dobos, Ph.D.
PROVIDER: PXD007588 | Pride | 2019-06-17
REPOSITORIES: Pride
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