Proteomics

Dataset Information

19

Proteomic response to the thaxtomin phytotoxin elicitor cellobiose and to the deletion of the cellulose utilization regulator CebR in Streptomyces scabies


ABSTRACT: Streptomyces scabies is a plant pathogen responsible for common scab disease on root and tuber crops. The transport of the cellulose by-products cellobiose and cellotriose into the cell induces the biosynthesis of the main phytotoxin thaxtomin A. These carbohydrates bind to the cis-acting sequences of the cellulose utilization repressor CebR and thereby induce the expression of the thaxtomin activator TxtR. We performed a shotgun proteomic experiment to compare the wild-type S. scabies 87-22 and its cebR null mutant in the absence or presence of cellobiose. The global intracellular response during virulent behaviour of this plant pathogen could be determined during this experiment.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Streptomyces scabiei 87.22  

TISSUE(S): Tissue Not Applicable To Dataset

DISEASE(S): Not Available

SUBMITTER: Sören Planckaert  

LAB HEAD: Bart Devreese

PROVIDER: PXD007820 | Pride | 2018-10-18

REPOSITORIES: Pride

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Publications

Proteomic Response to Thaxtomin Phytotoxin Elicitor Cellobiose and to Deletion of Cellulose Utilization Regulator CebR in Streptomyces scabies.

Planckaert Sören S   Jourdan Samuel S   Francis Isolde M IM   Deflandre Benoit B   Rigali Sébastien S   Devreese Bart B  

Journal of proteome research 20181010 11


Streptomyces scabies is responsible for common scab disease on root and tuber vegetables. Production of its main phytotoxin thaxtomin A is triggered upon transport of cellulose byproducts cellotriose and cellobiose, which disable the repression of the thaxtomin biosynthesis activator gene txtR by the cellulose utilization regulator CebR. To assess the intracellular response under conditions where S. scabies develops a virulent behavior, we performed a comparative proteomic analysis of wild-type  ...[more]

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