Proteomic response to the thaxtomin phytotoxin elicitor cellobiose and to the deletion of the cellulose utilization regulator CebR in Streptomyces scabies

ABSTRACT: Streptomyces scabies is a plant pathogen responsible for common scab disease on root and tuber crops. The transport of the cellulose by-products cellobiose and cellotriose into the cell induces the biosynthesis of the main phytotoxin thaxtomin A. These carbohydrates bind to the cis-acting sequences of the cellulose utilization repressor CebR and thereby induce the expression of the thaxtomin activator TxtR. We performed a shotgun proteomic experiment to compare the wild-type S. scabies 87-22 and its cebR null mutant in the absence or presence of cellobiose. The global intracellular response during virulent behaviour of this plant pathogen could be determined during this experiment.

INSTRUMENT(S):

ORGANISM(S): Streptomyces Scabiei 87.22

SUBMITTER: Sören Planckaert  

LAB HEAD: Bart Devreese

PROVIDER: PXD007820 | Pride | 2018-10-18

REPOSITORIES: Pride