Proteomics

Dataset Information

9

Beta-glucan is a major growth substrate for human gut bacteria related to Coprococcus eutactus


ABSTRACT: A clone encoding carboxymethylcellulase activity was isolated during functional screening of a human gut metagenomic library using Lactococcus lactis MG1363 as heterologous host. The insert sequence revealed a glycoside hydrolase family 9 (GH9) catalytic domain with sequence similarity to a gene from Coprococcus sp. ART55/1, which is closely related to Coprocococcus eutactus. Surveys of available genomes indicated a limited distribution of GH9 domains among dominant human colonic anaerobes. Genomes of two Coprococcus-related strains showed the presence of two GH9-encoding and four GH5-encoding genes, however, the strains did not appear to be able to degrade cellulose. Instead, they grew well on beta-glucans and one of the strains also showed growth on galactomannan, galactan and glucomannan. Gene expression and proteomic analysis of Coprococcus sp. ART55/1 grown on cellobiose, beta-glucan and lichenan led to a similar change in expression in comparison to glucose. On beta-glucan and lichenan only, one of the four GH5 genes was strongly upregulated. Growth on glucomannan lead to a transcriptional response of many genes, in particular a strong upregulation of glycoside hydrolases involved in mannan degradation. Thus, beta-glucans are a major growth substrate for species related to Coprococcus eutactus, with glucomannan and galactans alternative substrates for some strains.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Coprococcus sp. ART55/1  

TISSUE(S): Cell Culture

DISEASE(S): Not Available

SUBMITTER: Sophie Shaw  

LAB HEAD: Petra Louis

PROVIDER: PXD014174 | Pride | 2020-03-10

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
BG10C.raw Raw
BG1A.raw Raw
BG1C.raw Raw
Cb10A.raw Raw
Cb10B.raw Raw
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Publications

β-Glucan is a major growth substrate for human gut bacteria related to Coprococcus eutactus.

Alessi Anna M AM   Gray Victoria V   Farquharson Freda M FM   Flores-López Adriana A   Shaw Sophie S   Stead David D   Wegmann Udo U   Shearman Claire C   Gasson Mike M   Collie-Duguid Elaina S R ESR   Flint Harry J HJ   Louis Petra P  

Environmental microbiology 20200320 6


A clone encoding carboxymethyl cellulase activity was isolated during functional screening of a human gut metagenomic library using Lactococcus lactis MG1363 as heterologous host. The insert carried a glycoside hydrolase family 9 (GH9) catalytic domain with sequence similarity to a gene from Coprococcus eutactus ART55/1. Genome surveys indicated a limited distribution of GH9 domains among dominant human colonic anaerobes. Genomes of C. eutactus-related strains harboured two GH9-encoding and four  ...[more]

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