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Cdc42 phosphorylation study - Cdc42 Regulates Neuronal Polarity during Cerebellar Axon Formation and Glial-Guided Migration

ABSTRACT: Govek et al. demonstrate conditional loss of Cdc42 in cerebellar granule cell progenitors (GCPs) perturbs GCP polarity and impairs axon patterning, glial-guided migration, and cerebellar foliation. Phospho-proteomic analysis identified polarity and cytoskeletal proteins as affected targets in Cdc42 deficient GCPs.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: henrik molina

LAB HEAD: Mary E. Hatten

PROVIDER: PXD008148 | Pride | 2018-10-22

REPOSITORIES: Pride

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Cdc42 Regulates Neuronal Polarity during Cerebellar Axon Formation and Glial-Guided Migration.

Govek Eve-Ellen EE Wu Zhuhao Z Acehan Devrim D Molina Henrik H Rivera Keith K Zhu Xiaodong X Fang Yin Y Tessier-Lavigne Marc M Hatten Mary Elizabeth ME

iScience 20180301

CNS cortical histogenesis depends on polarity signaling pathways that regulate cell adhesion and motility. Here we report that conditional deletion of the Rho GTPase <i>Cdc42</i> in cerebellar granule cell precursors (GCPs) results in abnormalities in cerebellar foliation revealed by iDISCO clearing methodology, a loss of columnar organization of proliferating GCPs in the external germinal layer (EGL), disordered parallel fiber organization in the molecular layer (ML), and a failure to extend a ...[more]

PMID: 29888765

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