Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Homo Sapiens (human) Saccharomyces Cerevisiae (baker's Yeast)
TISSUE(S): Cell Culture
SUBMITTER: James Wright
LAB HEAD: Jyoti Choudhary
PROVIDER: PXD008624 | Pride | 2019-04-03
REPOSITORIES: Pride
Bakos Gábor G Yu Lu L Gak Igor A IA Roumeliotis Theodoros I TI Liakopoulos Dimitris D Choudhary Jyoti S JS Mansfeld Jörg J
Nature communications 20181114 1
Covalent modifications of proteins with ubiquitin and ubiquitin-like molecules are instrumental to many biological processes. However, identifying the E3 ligase responsible for these modifications remains a major bottleneck in ubiquitin research. Here, we present an E2-thioester-driven identification (E2~dID) method for the targeted identification of substrates of specific E2 and E3 enzyme pairs. E2~dID exploits the central position of E2-conjugating enzymes in the ubiquitination cascade and pro ...[more]