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CDKL5 substrates - Phosphoproteomic screening identifies physiological substrates of the CDKL5 kinase

ABSTRACT: Identification of CDKL5 substrates. Immuno-precipitated proteins were TMT labelled and analysed via LC-MS/MS.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Osteosarcoma

SUBMITTER: John Rouse

LAB HEAD: John Rouse

PROVIDER: PXD009327 | Pride | 2018-10-02

REPOSITORIES: Pride

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Phosphoproteomic screening identifies physiological substrates of the CDKL5 kinase.

Muñoz Ivan M IM Morgan Michael E ME Peltier Julien J Weiland Florian F Gregorczyk Mateusz M Brown Fiona Cm FC Macartney Thomas T Toth Rachel R Trost Matthias M Rouse John J

The EMBO journal 20180928 24

Mutations in the gene encoding the protein kinase CDKL5 cause a debilitating neurodevelopmental disease termed CDKL5 disorder. The impact of these mutations on CDKL5 function is poorly understood because the substrates and cellular processes controlled by CDKL5 are unclear. Here, we describe a quantitative phosphoproteomic screening which identified MAP1S, CEP131 and DLG5-regulators of microtubule and centrosome function-as cellular substrates of CDKL5. Antibodies against MAP1S phospho-Ser<sup>9 ...[more]

PMID: 30266825

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