Proteomics

Dataset Information

36

Proximity labeling and identification of the proteins that interact with Siglec-14 on THP-1 cells


ABSTRACT: Proteins that interact with Siglec-14 were identified by proximity labeling, followed by streptavidin affinity purification and proteomic analysis. MaxQuant software was used for the quantitative analysis of the proteins identified.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo sapiens  

TISSUE(S): Cell Culture

DISEASE(S): Not Available

SUBMITTER: Jimmy Huang  

LAB HEAD: Takashi Angata

PROVIDER: PXD009748 | Pride | 2018-11-04

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
EV_0928_171011-2.raw Raw
EV_1003_171011.raw Raw
F-sig14_0928_171011.raw Raw
F-sig14_1003_171011.raw Raw
MaxQuant_Output.zip Other
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Publications

Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses.

Huang Po-Chun Jimmy PJ   Low Penk-Yeir PY   Wang Iren I   Hsu Shang-Te Danny SD   Angata Takashi T  

The Journal of biological chemistry 20181030 51


Human sialic acid-binding immunoglobulin-like lectin 14 (Siglec-14) is a glycan-recognition protein that is expressed on myeloid cells, recognizes bacterial pathogens, and elicits pro-inflammatory responses. Although Siglec-14 is a transmembrane protein, a soluble form of Siglec-14 is also present in human blood. However, the mechanism that generates soluble Siglec-14 and what role this protein form may play remain unknown. Here, investigating the generation and function of soluble Siglec-14, we  ...[more]

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