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Peptidomics profiling of human intestine

ABSTRACT: The aim of the project is to generate a peptidomics map of gut hormone peptides along the gastrointestinal tract, starting with the stomach and including the duodenum, jejunum, ileum, ascending colon, sigmoid colon and rectum. The tissues would be collected after surgery and the peptide fraction extracted and anlysed by nano LC-MS to identify what peptide hormones are present. These data will then be used to compare against the human transcriptome, and also for comparison against equivent peptides from murine intestinal extracts.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stomach, Colon Ascendens, Jejunum, Duodenum, Rectum, Ileum, Colon Sigmoideum

SUBMITTER: Richard Kay

LAB HEAD: Fiona M Gribble

PROVIDER: PXD009788 | Pride | 2019-02-15

REPOSITORIES: Pride

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			Action	DRS
	17120501.mgf	Mgf
	17120501.pride.mgf.gz	Mgf
	17120501.raw	Raw
	17120502.mgf	Mgf
	17120502.pride.mgf.gz	Mgf

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1 - 5 of 125

Publications

Comparison of Human and Murine Enteroendocrine Cells by Transcriptomic and Peptidomic Profiling.

Roberts Geoffrey P GP Larraufie Pierre P Richards Paul P Kay Richard G RG Galvin Sam G SG Miedzybrodzka Emily L EL Leiter Andrew A Li H Joyce HJ Glass Leslie L LL Ma Marcella K L MKL Lam Brian B Yeo Giles S H GSH Scharfmann Raphaël R Chiarugi Davide D Hardwick Richard H RH Reimann Frank F Gribble Fiona M FM

Diabetes 20190207 5

Enteroendocrine cells (EECs) produce hormones such as glucagon-like peptide 1 and peptide YY that regulate food absorption, insulin secretion, and appetite. Based on the success of glucagon-like peptide 1-based therapies for type 2 diabetes and obesity, EECs are themselves the focus of drug discovery programs to enhance gut hormone secretion. The aim of this study was to identify the transcriptome and peptidome of human EECs and to provide a cross-species comparison between humans and mice. By R ...[more]

PMID: 30733330

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Peptidomics profiling of murine intestine

Project description:The aim of the project is to generate a peptidomics map of gut hormone peptides along the gastrointestinal tract, starting with the stomach and including the duodenum, jejunum, ileum, colon and rectum. The tissues would be collected after surgery and the peptide fraction extracted and anlysed by nano LC-MS to identify what peptide hormones are present. These data will then be used to compare against the murine transcriptome, and also for comparison against equivent peptides from human intestinal extracts.

2019-02-15 | PXD009796 | Pride

Multi-omic biogeography of the gastrointestinal microbiota of a pre-weaned lamb

Project description:Here, we used reverse-phase liquid chromatography-coupled tandem mass spectrometry to study the pre-weaned lamb proteome and metaproteome in ten different gastrointestinal tracts: rumen, reticulum, omasum, abomasum, duodenum, jejunum, ileum, cecum, colon, and rectum.

2017-12-14 | PXD008192 | Pride

Gene expression profile of epithelial cells from five regions of human intestinal.

Project description:Data independent analysis of proteome of patient derived epithelial cell from five regions, duodenum, jejunum, ileum, colon and rectum.

2023-05-08 | PXD037197 | JPOST Repository

Characterization of Guca2a expressing cells in the mouse intestine

Project description:We report here the transcriptome of sorted cells from a Guca2a promoter GFP mouse model, positve and negative cells for GFP from 4 different regions of the GI tract, duodenum (top 3cm), jejunum (intermediate small intestine), ileum (bottom 10 cm) and colon +rectum from 3 different mice. This data allows the characterization of the cells expressing Guca2a at the trancriptome level, revealling expression of different cell markers and showing diversity of Guca2a producing cells along the GI tract.

2021-03-01 | GSE128117 | GEO

In vivo gene expression profile of human intestinal epithelial cells: from the viewpoint of drug metabolism and pharmacokinetics

Project description:Orally administered drugs are absorbed and metabolized in the intestine. In order to accurately predict pharmacokinetics in the intestine, it is essential to understand the intestinal expression profiles of genes related to drug absorption, distribution, metabolism, and excretion (ADME). However, in many previous studies, gene expression analysis in the intestine has been carried out using specimens from cancer patients. In this study, in order to obtain a more accurate gene expression profile, biopsy samples were collected from 14 patients under endoscopic observation and RNA-seq analysis was performed. Gene expression analysis of drug metabolizing enzymes (CYPs), non-CYP enzymes, nuclear receptors, drug conjugating enzymes (UGTs and SULTs), and apical and basolateral drug transporters was performed in biopsy samples from the duodenum, ileum, colon, and rectum. The proportions of the CYPs expressed in the ileum were 25% (CYP3A4), 19% (CYP2C18), and 14% (CYP3A5). CYP3A4 and CYP2C19 were highly expressed in the duodenum and ileum, but not in the colon and rectum. In the ileum, apical transporters such as P-gp, PEPT1, BCRP, MRP2, and ASBT were strongly expressed, and the expression levels of P-gp and ASBT in the ileum were higher than those in other regions. In the ileum, basolateral transporters such as OSTα, OSTβ, and MRP3 were strongly expressed. Furthermore, we identified specific markers for the duodenum, ileum, colon, and rectum by conducting comprehensive gene expression analysis. We succeeded in obtaining gene expression profiles of drug ADME-related genes in vivo human intestinal epithelial cells. We expect that this information would be useful for accurate prediction of the pharmacokinetics of oral drugs.

2021-04-30 | GSE156453 | GEO

Genomic profile of the mouse intestine

Project description:8 week-old male Hsd:ICR(CD-1) mice were fed ad libitum a standard chow (Harlan Teklad diet 2018S) and housed in groups of five. Animals were then divided into 3 pools (n=10) and sacrificed. The small intestine was extracted and divided into three sections, where the first 2-3 cm after the stomach comprised the duodenum, the middle third the jejunum, and the section before the ileo-ceco-colic junction comprised the ileum. Colon was not divided into proximal and distal sections and was treated as a single intestinal section. Keywords: other

2003-12-04 | GSE849 | GEO

RNA-seq of mouse small intestinal epithelial cells sorted from duodenum, jejunum and ileum

Project description:Results of RNA-seq of normal C57BL/6 small intestinal epithelial cells sorted from duodenum, jejunum and ileum separately. Samples are named as follow; mouse replicate number-duodenum(1), jejunum(2) or ileum(3). For example, 1-1, 1-2 and 1-3 representing duodenum, jejunum and ileum respectively from mouse replicate number 1.

2021-01-21 | E-MTAB-9744 | biostudies-arrayexpress

MicroRNAomes of cattle intestinal tissues revealed possible miRNA regulated mechanisms involved in Escherichia coli O157 fecal shedding

Project description:The current study aimed to investigate whether bovine non-coding RNA play a role in regulating E. coli O157 shedding through studying miRNAomes of the whole gastrointestinal tract including duodenum, proximal jejunum, distal jejunum, cecum, spiral colon, descending colon and rectum. The number of miRNAs detected in each intestinal region ranged from 390 ± 13 to 413 ± 49. Compared between SS and NS, the number of differentially expressed (DE) miRNAs ranged from one to eight, and through the whole gut, seven miRNAs were up-regulated and seven were down-regulated in SS. The distal jejunum and rectum were the regions where the most DE miRNAs were identified (8 and 7, respectively). Functional analysis indicated that the bta-miR-378b, bta-miR-2284j and bta-miR-2284d which were down-regulated in both distal jejunum and rectum of SS, the bta-miR-2887 which was down-regulated in rectum of SS, as well as the bta-miR-211 and bta-miR-29d-3p which were up-regulated in rectum of SS were potentially regulatory to host immune functions, including hematological system development and immune cell trafficking. Our findings suggest that the alternation of miRNA expression in the gut of SS may lead to differential regulation in immune functions involved in E. coli O157 super-shedding in cattle.

2018-10-30 | GSE121916 | GEO

MicroRNAomes of cattle intestinal tissues revealed possible miRNA regulated mechanisms involved in Escherichia coli O157 fecal shedding

2018-12-01 | GSE96973 | GEO

Gene expression of human small intestine generated by biopsy specimens

Project description:The entire small intestine was obseved by balloon endoscopy. Biopsy specimens were taken from jejunum, ileum and colon, respectively.

2015-06-11 | GSE69762 | GEO

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