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Peptidomics profiling of murine intestine

ABSTRACT: The aim of the project is to generate a peptidomics map of gut hormone peptides along the gastrointestinal tract, starting with the stomach and including the duodenum, jejunum, ileum, colon and rectum. The tissues would be collected after surgery and the peptide fraction extracted and anlysed by nano LC-MS to identify what peptide hormones are present. These data will then be used to compare against the murine transcriptome, and also for comparison against equivent peptides from human intestinal extracts.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Stomach, Jejunum, Duodenum, Rectum, Colon, Ileum

SUBMITTER: Richard Kay

LAB HEAD: Fiona M Gribble

PROVIDER: PXD009796 | Pride | 2019-02-15

REPOSITORIES: Pride

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Important Role of the GLP-1 Axis for Glucose Homeostasis after Bariatric Surgery.

Larraufie Pierre P Roberts Geoffrey P GP McGavigan Anne K AK Kay Richard G RG Li Joyce J Leiter Andrew A Melvin Audrey A Biggs Emma K EK Ravn Peter P Davy Kathleen K Hornigold David C DC Yeo Giles S H GSH Hardwick Richard H RH Reimann Frank F Gribble Fiona M FM

Cell reports 20190201 6

Bariatric surgery is widely used to treat obesity and improves type 2 diabetes beyond expectations from the degree of weight loss. Elevated post-prandial concentrations of glucagon-like peptide 1 (GLP-1), peptide YY (PYY), and insulin are widely reported, but the importance of GLP-1 in post-bariatric physiology remains debated. Here, we show that GLP-1 is a major driver of insulin secretion after bariatric surgery, as demonstrated by blocking GLP-1 receptors (GLP1Rs) post-gastrectomy in lean hum ...[more]

PMID: 30726726

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