Ontology highlight
ABSTRACT:
INSTRUMENT(S): Synapt MS
ORGANISM(S): Escherichia Coli
SUBMITTER: Chloe Martens
LAB HEAD: Argyris Politis
PROVIDER: PXD011060 | Pride | 2018-10-16
REPOSITORIES: Pride
Martens Chloe C Shekhar Mrinal M Borysik Antoni J AJ Lau Andy M AM Reading Eamonn E Tajkhorshid Emad E Booth Paula J PJ Politis Argyris A
Nature communications 20181008 1
Secondary transporters undergo structural rearrangements to catalyze substrate translocation across the cell membrane - yet how such conformational changes happen within a lipid environment remains poorly understood. Here, we combine hydrogen-deuterium exchange mass spectrometry (HDX-MS) with molecular dynamics (MD) simulations to understand how lipids regulate the conformational dynamics of secondary transporters at the molecular level. Using the homologous transporters XylE, LacY and GlpT from ...[more]