Browse
Submit Data
Databases
API
Help

Dataset Information

244 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteomic analysis of roots of Arabidopsis plda1 mutants

ABSTRACT: PLDa1 is a phospholipid hydrolyzing enzyme playing multiple regulatory roles in stress response in plants. Its signaling activity is mediated via the PLDa1 dependent phosphatidic acid production, by its capacity to bind and modulate the G-protein complex or by interaction with other proteins. Relative quantitative proteomic analysis of two tDNA insertion Arabidopsis plda1 mutants has been performed. Remarkably, PLDa1 deficiency caused differential regulation of many proteins which form protein complexes.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Root

SUBMITTER: Tibor Pechan

LAB HEAD: Tibor Pechan

PROVIDER: PXD011196 | Pride | 2019-01-21

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Publications

Shot-Gun Proteomic Analysis on Roots of Arabidopsis <i>pldα1</i> Mutants Suggesting the Involvement of PLDα1 in Mitochondrial Protein Import, Vesicular Trafficking and Glucosinolate Biosynthesis.

Takáč Tomáš T Šamajová Olga O Vadovič Pavol P Pechan Tibor T Šamaj Jozef J

International journal of molecular sciences 20181226 1

Phospholipase Dα1 (PLDα1) belongs to phospholipases, a large phospholipid hydrolyzing protein family. PLDα1 has a substrate preference for phosphatidylcholine leading to enzymatic production of phosphatidic acid, a lipid second messenger with multiple cellular functions. PLDα1 itself is implicated in biotic and abiotic stress responses. Here, we present a shot-gun differential proteomic analysis on roots of two Arabidopsis <i>pldα1</i> mutants compared to the wild type. Interestingly, PLDα1 defi ...[more]

PMID: 30587782

Similar Datasets

Proteomic analysis of Arabidopsis plda1 mutants

Project description:PLDa1 is a phospholipid hydrolyzing enzyme playing multiple regulatory roles in stress response in plants. Its signaling activity is mediated via the PLDa1 dependent phosphatidic acid production, by its capacity to bind and modulate the G-protein complex or by interaction with other proteins. Relative quantitative proteomic analysis of two tDNA insertion Arabidopsis plda1 mutants has been performed. Remarkably, PLDa1 deficiency caused differential regulation of many proteins which form protein complexes.

2019-03-12 | PXD009725 | Pride

Functional characterization of Taf4b in Arabidopsis thaliana

Project description:Expression analysis was performed with two TDNA insertion mutants of taf4b i.e; taf4bprm (TDNA insertion in promoter region) and taf4bint (TDNA insertion in intronic region), Taf4b overexpression lines, taf4bprmcpr5 double mutant lines (Double mutant was generated by crossing taf4bprm with cpr5) and Col-0 in normal condition as well as with taf4bprm mutant and Col-0 infected with fungi AB (Alternaria brassicicola) and bacteria ES4 (Pseudomonas syringae pv.maculicola ES4326 ) in different perspectives. Affymatrix expression analysis was executed to provide mechanistic details of regulation of genes by Taf4b in plants.

2016-12-01 | GSE45690 | GEO

Transcription profiling of Arabidopsis CYP98A3 knockout mutant

Project description:TDNA insertion mutants in CYP98A3 were produced by K. Feldmann, and studied through transcriptomic analysis against the wild type WS (specific insertion was checked by PCR and segregation analysis). Characterization of knockout mutants for P450s can be very helpful to fully understand the function of the disrupted gene, and is expected to generate information about the molecular basis of mutants phenotype.

2005-04-29 | E-MEXP-198 | biostudies-arrayexpress

HAP5 Triple Mutant vs. Constans Mutant

Project description:Aims: To compare the transcriptional profiles of an AtHAP5 (AtNF-YC, AtCBF-C) triple mutant vs. a Constans mutant. Background Information on Mutants: AtHAP5 triple mutant created by cross between the following SALK TDNA inserts: SALK_034838, SALK_058903, SALK_032163; these correspond to At1G54830, At1G08970, At5G63470, respectively. co-SAIL TDNA insertion SAIL_24_H04 corresponds to AT5G15840. Tissue: Above-ground whole plants grown for 10 days, under 16 hr light 8 hrs dark at constant 22C. Tissue collected 14 hrs after lights on. RT-PCR analysis on test RNA confirmed FT levels reduced in mutants lines compared to wild-type. 9 samples were used in this experiment.

2010-04-28 | E-GEOD-20258 | biostudies-arrayexpress

Microarray experiment on Arabidopsis roots infected with M. incognita for mutant lines of DSC1 and WRKY19 compared to Col-0

Project description:In this experiment we compare the effect of T-DNA insertion in DSC1 and WRKY19 during M. incognita infection. Where we compare the infected roots to uninfected roots at dpi 0 as the start of the infection and 7dpi, 7 days after inoculation. Both mutants are compared against Col-0 the wildtype plant.

2019-06-01 | E-MTAB-6897 | biostudies-arrayexpress

HIV-1 integrase variants retarget viral integration and are associated with disease progression in a chronic infection cohort.

Project description:Distinct integration patterns of different retroviruses have puzzled virologists for over 20 years. The viral integrase (IN), as part of the intasome complex, docks onto the target DNA (tDNA) and catalyzes the insertion of the viral genome into the host chromatin. We identified retroviral IN amino acids directly contacting tDNA bases and affecting the local integration site sequence biases. These residues also determine the propensity of the virus to integrate into flexible tDNA sequences. Remarkably, natural polymorphisms INS119G and INR231G retarget viral integration away from gene dense regions, without affecting the interaction with the lentiviral tethering cofactor LEDGF/p75 (PSIP1). Precisely these variants were associated with rapid disease progression in a chronic HIV-1 subtype C infection cohort. These findings link integration site selection to virulence and viral evolution but also to the host immune response and antiretroviral therapy, since HIV-1 IN119 is under selection by HLA alleles and integrase inhibitors. LEDGF/p75 (PSIP1) ChIP-Seq using A300-848 antibody (recognizes p75 isoform) and input control in primary CD4+ T-cells

2015-01-26 | E-GEOD-61003 | biostudies-arrayexpress

HAP5 Triple Mutant vs. Constans Mutant

2010-04-20 | GSE20258 | GEO

HIV-1 integrase variants retarget viral integration and are associated with disease progression in a chronic infection cohort.

2015-01-26 | GSE61003 | GEO

What is the transcriptomic contrast between a TDNA-mutant and its respective wild-type from young Arabidopsis rosette?

Project description:ra15-07_burxcol_465 - burxcol-465 - What is the transcriptomic contrast between a TDNA-mutant and its respective wild-type from young rosette grown in precisely-controlled conditions on our phenotyping robot (Phenoscope)? - The candidate gene (AT1G36310) was identified through QTL mapping and cloning for rosette leaf growth trait in a Bur x Col RIL set. It corresponds to a medium effect on growth and a major effect on other traits (leaf colour and pigments). We are now exploiting an independent TDNA-insertion line (SALK_135308) in the Col-0 background) to study its effect in a simple genetic context. This is a simple comparison between homozygous T-DNA (2 lines) and their respective WT (2 lines).

2017-02-01 | GSE93513 | GEO

Arabidopsis MED8 functions in oxidative stress

Project description:In this work, we describe a TDNA insertion mutant for Mediator complex subunit 8 (MED8) that regulates the oxidative stress responses. Wild-type Col-0 and med8 seedlings were growth under control condition or oxidative stress (induced by methyl viologen treatment) and were subjected to RNA-seq profiling. Total mRNA fractions were isolated and subjected to signle-end deep sequencing (approx.30M reads/sample) to reveal differential expression between genotypes and conditions.

2021-03-14 | GSE141547 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data