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Quantitative Proteomics analysis of LX-2 Responses to Irradiation and TGF-β1 Description

ABSTRACT: The human hepatic stellate cell line LX2 was treated with 8 Gy X-ray irradiation and/or 2ng/ml recombinant human TGF-β1. The iTRAQ-based high throughput quantitative proteomic approach was used to obtain a comprehensive view of the protein ensembles affected by irradiation and/or TGF-β1 treatment on LX2. This study provides clues for further investigation of the mechanisms behind radiation-induced liver fibrosis.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatic Stellate Cell

SUBMITTER: Baoying Yuan

LAB HEAD: Zhaochong Zeng

PROVIDER: PXD011242 | Pride | 2018-12-04

REPOSITORIES: Pride

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Proteomic Profiling of Human Hepatic Stellate Cell Line LX2 Responses to Irradiation and TGF-β1.

Yuan Baoying B Chen Yuhan Y Wu Zhifeng Z Zhang Li L Zhuang Yuan Y Zhao Xiaomei X Niu Hao H Cheng Jason Chia-Hsien JC Zeng Zhaochong Z

Journal of proteome research 20181210 1

Hepatic stellate cells (HSCs) are the main target of radiation damage and primarily contribute to the development of radiation-induced liver fibrosis. However, the molecular events underlying the radiation-induced activation of HSCs are not fully elucidated. In the present study, human HSC line LX2 was treated with X-ray irradiation and/or TGF-β1, and profibrogenic molecules were evaluated. The iTRAQ LC-MS/MS technology was performed to identify global protein expression profiles in LX2 followin ...[more]

PMID: 30489086

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