Mass-spectrometry-based analysis of the secretome of M. tuberculosis mutants
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Mycobacterium Tuberculosis H37rv
TISSUE(S): Cell Culture, Cell Line Cell
DISEASE(S): Pulmonary Tuberculosis
SUBMITTER: 
Andrej Benjak
LAB HEAD: Stewart Thomas Cole
PROVIDER: PXD011466 | Pride | 2018-12-10
REPOSITORIES: Pride
Publications
EspL is essential for virulence and stabilizes EspE, EspF and EspH levels in Mycobacterium tuberculosis.
Sala Claudia C Odermatt Nina T NT Soler-Arnedo Paloma P Gülen Muhammet F MF von Schultz Sofia S Benjak Andrej A Cole Stewart T ST
PLoS pathogens 20181220 12
The ESX-1, type VII, secretion system represents the major virulence determinant of Mycobacterium tuberculosis, one of the most successful intracellular pathogens. Here, by combining genetic and high-throughput approaches, we show that EspL, a protein of 115 amino acids, is essential for mediating ESX-1-dependent virulence and for stabilization of EspE, EspF and EspH protein levels. Indeed, an espL knock-out mutant was unable to replicate intracellularly, secrete ESX-1 substrates or stimulate in ...[more]
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