Proteomics

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Proteomics of venom from Trimeresurus malabaricus


ABSTRACT: This project mainly aims to characterize the complex toxic components present in the venom of Trimeresurus malabaricus (Malabar pit viper). Since Trimeresurus malabaricus (Malabar pit viper) species are mainly inhabited to plantation crop areas, its envenomation is a serious threat to the human population thriving in these zones, especially to the plantation workers. Therefore, exploring the venom proteome of Malabar pit viper is decisive to develop and design new antivenom and therapeutics against its envenomation. As described in this study, applying various orthogonal separation strategies helped in dissecting venom constituents of Trimeresurus malabaricus and is the first comprehensive attempt in revealing the complex venom profile of Malabar pit viper through proteomics approaches incorporating multiple database searches. In order to achieve this the crude venom components were resolved on a 12% SDS page. Further each of the bands were subjected to in-gel trypsin digestion. The crude venom was also subjected to ion-exchange chromatography separation. The obtained fractions were subjected to in-solution trypsin digestion. All the digested peptides were then subjected to Q-TOF LC-MS/MS analysis.

INSTRUMENT(S): 6540 Q-TOF LC/MS

ORGANISM(S): Serpentes

SUBMITTER: Sudarslal Sadasivan Nair  

LAB HEAD: Sudarslal Sadasivan Nair

PROVIDER: PXD011673 | Pride | 2020-01-24

REPOSITORIES: Pride

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Publications

Delineating the venom toxin arsenal of Malabar pit viper (Trimeresurus malabaricus) from the Western Ghats of India and evaluating its immunological cross-reactivity and in vitro cytotoxicity.

Vanuopadath Muralidharan M   Shaji Sanu Korumadathil SK   Raveendran Dileepkumar D   Nair Bipin Gopalakrishnan BG   Nair Sudarslal Sadasivan SS  

International journal of biological macromolecules 20200123


The venom protein components of Malabar pit viper (Trimeresurus malabaricus) were identified by combining SDS-PAGE and ion-exchange chromatography pre-fractionation techniques with LC-MS/MS incorporating Novor and PEAKS-assisted de novo sequencing strategies. Total 97 proteins that belong to 16 protein families such as L-amino acid oxidase, metalloprotease, serine protease, phospholipase A<sub>2</sub>, 5'-nucleotidase, C-type lectins/snaclecs and disintegrin were recognized from the venom of a s  ...[more]

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