Dataset Information

Discovery and structure of the mitoribosomal SSU assemblosome in trypanosomes

ABSTRACT: Data-dependent LC-MS/MS was used to generate a protein inventory of an affinity-purified assembly intermediate of the small subunit (SSU) of the Trypanosoma brucei mitochondrial ribosome. The identified proteins (mitoribosomal proteins and putative maturation factors) were then used to assist in solving the structure of the SSU assemblosome by cryo-electron microscopy.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Trypanosoma Brucei Brucei Treu927

SUBMITTER: Alexander Leitner

LAB HEAD: Nenad Ban

PROVIDER: PXD011988 | Pride | 2019-09-13

REPOSITORIES: Pride

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Publications

Mitoribosomal small subunit biogenesis in trypanosomes involves an extensive assembly machinery.

Saurer Martin M Ramrath David J F DJF Niemann Moritz M Calderaro Salvatore S Prange Céline C Mattei Simone S Scaiola Alain A Leitner Alexander A Bieri Philipp P Horn Elke K EK Leibundgut Marc M Boehringer Daniel D Schneider André A Ban Nenad N

Science (New York, N.Y.) 20190901 6458

Mitochondrial ribosomes (mitoribosomes) are large ribonucleoprotein complexes that synthesize proteins encoded by the mitochondrial genome. An extensive cellular machinery responsible for ribosome assembly has been described only for eukaryotic cytosolic ribosomes. Here we report that the assembly of the small mitoribosomal subunit in <i>Trypanosoma brucei</i> involves a large number of factors and proceeds through the formation of assembly intermediates, which we analyzed by using cryo-electron ...[more]

PMID: 31515389

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Project description:BACKGROUND & AIMS: Recent studies revealed that hemoglobin is expressed in some non-erythrocytes and it suppresses oxidative stress when overexpressed. Oxidative stress plays a critical role in the pathogenesis of non-alcoholic steatohepatitis (NASH). This study was to investigate whether hemoglobin is expressed in hepatocytes and how it is related to oxidative stress in NASH patients. METHODS: Microarray was performed to identify differentially expressed genes in NASH. Quantitative real time PCR (qRT-PCR) was used to examine gene expression levels. Western blotting and immunofluorescence staining were employed to examine hemoglobin proteins. Flow cytometry was used to measure intracellular oxidative stress. RESULTS: Analysis of microarray gene expression data has revealed a significant increase in the expression of hemoglobin alpha (HBA1) and beta (HBB) in liver biopspies from NASH patients. Increased hemoglobin expression in NASH was validated by qRT-PCR. However, the expression of erythrocyte specific marker genes such as SPTA, SPTB, GYPA, GATA1, and ALAS2 did not change, indicating that increased hemoglobin expression in NASH was not from erythropoiesis, but could result from increased expression in hepatocytes. Immunofluorescence staining demonstrated positive HBA1 and HBB expression in the hepatocytes of NASH livers. Hemoglobin expression was also observed in human hepatocellular carcinoma HepG2 cell line. Furthermore, treatment with hydrogen peroxide, a known oxidative stress inducer, induced a dose dependent increase in HBA1 expression in HepG2 cells. Intriguingly, forced hemoglobin expression suppressed oxidative stress. CONCLUSIONS: Oxidative stress upregulates hemoglobin expression in hepatocytes. Suppression of oxidative stress by hemoglobin could be a mechanism to protect hepatocytes from oxidative damage. These findings suggest that hemoglobin is an inducible antioxidant in hepatocytes in response to increased oxidative stress as found in NASH livers. Twelve biopsy diagnosed NASH patients were included in this study. For control groups, total RNA from 5 different subjects were purchased from ADMET. These subjects are free from liver disease.

Dataset Information

Discovery and structure of the mitoribosomal SSU assemblosome in trypanosomes

Publications

Mitoribosomal small subunit biogenesis in trypanosomes involves an extensive assembly machinery.

Similar Datasets

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