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Proteome analysis of xylose metabolism in Rhodotorula toruloides during lipid production

ABSTRACT: Our study is the first investigation of proteome of oleaginous yeast Rhotodorula toruloides during conversion of xylose into lipids. We performed comparative proteomics of R. toruloides during cultivation of two carbon sources: glucose and xylose. Proteome analysis revealed significantly lower levels of ribosomal proteins and translation associated factors in xylose-grown cells as compared to glucose-grown cells. We showed that proteins involved in sugar transport, phospholipid and leucine biosynthesis as well as peroxisomal beta-oxidation and oxidative stress response were differentially regulated in response to carbon source.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Rhodotorula Toruloides

TISSUE(S): Vegetative Cell (sensu Fungi)

SUBMITTER: Ievgeniia Tiukova

LAB HEAD: Jens Nielsen

PROVIDER: PXD012332 | Pride | 2019-08-08

REPOSITORIES: Pride

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Proteome analysis of xylose metabolism in <i>Rhodotorula toruloides</i> during lipid production.

Tiukova Ievgeniia A IA Brandenburg Jule J Blomqvist Johanna J Sampels Sabine S Mikkelsen Nils N Skaugen Morten M Arntzen Magnus Ø MØ Nielsen Jens J Sandgren Mats M Kerkhoven Eduard J EJ

Biotechnology for biofuels 20190604

<h4>Background</h4><i>Rhodotorula toruloides</i> is a promising platform organism for production of lipids from lignocellulosic substrates. Little is known about the metabolic aspects of lipid production from the lignocellolosic sugar xylose by oleaginous yeasts in general and <i>R. toruloides</i> in particular. This study presents the first proteome analysis of the metabolism of <i>R. toruloides</i> during conversion of xylose to lipids.<h4>Results</h4><i>Rhodotorula toruloides</i> cultivated o ...[more]

PMID: 31171938

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