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DSSO,Xlink,LC-MSMS - DSSO as linker, study of protein-protein interaction by LC-MSMS

ABSTRACT: DSSO as linker, study of protein-protein interaction by LC-MSMS

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Streptococcus Pyogenes Stab901

TISSUE(S): B Cell

DISEASE(S): Disease Free

SUBMITTER: Wenzhang Chen

LAB HEAD: Wenzhang Chen

PROVIDER: PXD012466 | Pride | 2020-04-15

REPOSITORIES: Pride

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Allosteric inhibition of CRISPR-Cas9 by bacteriophage-derived peptides.

Cui Yan-Ru YR Wang Shao-Jie SJ Chen Jun J Li Jie J Chen Wenzhang W Wang Shuyue S Meng Bing B Zhu Wei W Zhang Zhuhong Z Yang Bei B Jiang Biao B Yang Guang G Ma Peixiang P Liu Jia J

Genome biology 20200226 1

<h4>Background</h4>CRISPR-Cas9 has been developed as a therapeutic agent for various infectious and genetic diseases. In many clinically relevant applications, constitutively active CRISPR-Cas9 is delivered into human cells without a temporal control system. Excessive and prolonged expression of CRISPR-Cas9 can lead to elevated off-target cleavage. The need for modulating CRISPR-Cas9 activity over time and dose has created the demand of developing CRISPR-Cas off switches. Protein and small molec ...[more]

PMID: 32102684

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