A chemical strategy for protease substrate profiling

ABSTRACT: Here, we introduce a simple method, termed CHOPS, for the discovery of protease substrates. CHOPS exploits a 2-pyridinecarboxaldehyde (2PCA)-biotin probe, which selectively biotinylates protein N-termini except those with proline in the second position. CHOPS can, in theory, discover substrates for any protease, but is particularly well-suited to discover canonical DPP substrates, as cleaved but not intact DPP substrates can be identified by gel electrophoresis or mass spectrometry. This work delineates a practical technology for identifying protease substrates, which we anticipate will complement available “N-terminomic” approaches.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Monocyte

DISEASE(S): Acute Leukemia

SUBMITTER: Andrew Griswold  

LAB HEAD: Daniel A. Bachovchin

PROVIDER: PXD013019 | Pride | 2019-04-23

REPOSITORIES: Pride