The interactome of the clathrin adaptor AP2 is modulated by the phosphorylation status of its µ2 subunit.

ABSTRACT: The clathrin adaptor AP2 is phosphorylated at a single site (Thr156) of its µ2 subunit. To gain insight how phosphorylation influences the interactome of AP2, we used human RPE cells which that were treated with LP-935509, an inhibitor of the adaptor-associated kinase AAK1. Kinase inhibition and loss of µ2 phosphorylation was verified with phospho-specific antibodies in westernblots of total cell extracts. We then used SILAC labelled cells and immune-isolated AP-2 and subjected bound proteins to mass spectrometry to identify all bound proteins and to determine phosphorylation-dependent changes in the interactome.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Retinal Pigment Epithelial Cell

SUBMITTER: Stefan Hoening  

LAB HEAD: Stefan Höning

PROVIDER: PXD013468 | Pride | 2019-11-12

REPOSITORIES: Pride