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Migrasomes provide regional cues for organ morphogenesis during zebrafish gastrulation

ABSTRACT: Migrasomes are recently identified vesicular organelles that form on retraction fibers behind migrating cells, cellular contents are released from migrasome by a process named migracytosis. The function of migrasomes in living organisms is unknown. Here we show that migrasomes are formed during zebrafish gastrulation, signaling molecules such as chemokines are enriched in migrasomes. Migrasomes are clustered on spatially restricted area in embryo where they provide regional cues for organ morphogenesis. Our study shown migrasome is signaling organelles which integrate spatial and specific biochemical information to coordinate migrating cells in complex biological processes such as morphogenesis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Danio Rerio (zebrafish) (brachydanio Rerio)

TISSUE(S): Embryo

SUBMITTER: Dong Jiang

LAB HEAD: Li Yu

PROVIDER: PXD013835 | Pride | 2019-06-03

REPOSITORIES: Pride

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Migrasomes provide regional cues for organ morphogenesis during zebrafish gastrulation.

Jiang Dong D Jiang Zheng Z Lu Di D Wang Xuan X Liang Haisha H Zhang Junfeng J Meng Yaping Y Li Ying Y Wu Danni D Huang Yuwei Y Chen Yuling Y Deng Haiteng H Wu Qing Q Xiong Jingwei J Meng Anming A Yu Li L

Nature cell biology 20190801 8

Migrasomes are recently identified vesicular organelles that form on retraction fibres behind migrating cells. Whether migrasomes are present in vivo and, if so, the function of migrasomes in living organisms is unknown. Here, we show that migrasomes are formed during zebrafish gastrulation and signalling molecules, such as chemokines, are enriched in migrasomes. We further demonstrate that Tspan4 and Tspan7 are required for migrasome formation. Organ morphogenesis is impaired in zebrafish MZtsp ...[more]

PMID: 31371827

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Project description:Migrasomes provide regional cues for organ morphogenesis during zebrafish gastrulation

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