Proteomic and metabolomic analysis of Azospirillum brasilense ntrC mutant under high and low nitrogen conditions

ABSTRACT: Azospirillum brasilense is a free-living diazothophic bacterium that associates with important grasses and cereals, promoting plant growth and increasing crop productivity. The nitrogen metabolism in this organism is tightly regulated by the availability of nitrogen and by the Ntr regulatory system. This system comprises the GlnD protein, which is capable of adding uridylyl groups to the PII proteins, GlnB and GlnZ, under limiting nitrogen levels. Under such conditions, the histidine kinase NtrB cannot interact with GlnB and phosphorylate NtrC. When phosphorylated, NtrC is a transcriptional activator of genes involved in metabolism of alternative nitrogen sources. Considering the key role of NtrC in nitrogen metabolism in Azospirillum brasilense, in this work we evaluated the proteomic and metabolomic profiles of the wild type FP2 strain and its mutant ntrC grown under high and low nitrogen. Analysis of the integrated data identifies, novel NtrC targets, including proteins involved in the response against oxidative stressnm (i.e., glutathione S-transferase and hydroperoxide resistance protein,), underlining the importance of NtrC to bacterial survival under oxidative stress conditions.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Azospirillum Brasilense Fp2

SUBMITTER: Gustavo De Souza  

LAB HEAD: Emmanuel M Souza

PROVIDER: PXD014120 | Pride | 2019-10-14

REPOSITORIES: Pride