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Human liver homogenate, liver microsomes, low-speed centrifugation pellet and hepatocytes LC-MS/MS from 15 matched donors

ABSTRACT: LC-MS/MS-based label-free proteomics of human liver homogenate, human liver microsomes, and human hepatocytes from 15 matched donors used to compare and understand differences in two major in vitro systems used for drug metabolism studies

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Liver

SUBMITTER: Christine Wegler

LAB HEAD: Per Artursson

PROVIDER: PXD014131 | Pride | 2021-03-29

REPOSITORIES: Pride

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Influence of Proteome Profiles and Intracellular Drug Exposure on Differences in CYP Activity in Donor-Matched Human Liver Microsomes and Hepatocytes.

Wegler Christine C Matsson Pär P Krogstad Veronica V Urdzik Jozef J Christensen Hege H Andersson Tommy B TB Artursson Per P

Molecular pharmaceutics 20210319 4

Human liver microsomes (HLM) and human hepatocytes (HH) are important in vitro systems for studies of intrinsic drug clearance (CLint) in the liver. However, the CLint values are often in disagreement for these two systems. Here, we investigated these differences in a side-by-side comparison of drug metabolism in HLM and HH prepared from 15 matched donors. Protein expression and intracellular unbound drug concentration (Kpuu) effects on the CLint</sub ...[more]

PMID: 33739838

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