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α-arrestin-dependent endocytosis after cycloheximide treatment

ABSTRACT: To identify new plasma membrane (PM) proteins that require α-arrestins for endocytosis, we performed an iTRAQ-based quantitative proteomic screening. We analysed and compared the PM proteome for each arrestin knockout (art∆) strain to a WT strain after a 90 min cycloheximide (CHX) treatment. CHX is a translation inhibitor and can trigger the endocytosis of several transporters in a substrate-independent manner. We searched for proteins that underwent CHX-induced endocytosis in a WT strain but that remained stabilized at the PM in an art∆ strain.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Jérôme Savocco

LAB HEAD: Pierre Morsomme

PROVIDER: PXD014695 | Pride | 2019-09-25

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20180822_first-screening_S1R1_new-db.group	Other
	20180822_first-screening_S1R1_new-db_MGFPeaklist.mgf	Mgf
	20180822_first-screening_S1R2_new-db.group	Other
	20180822_first-screening_S1R2_new-db_MGFPeaklist.mgf	Mgf
	20180822_first-screening_S1R3_new-db.group	Other

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