Dataset Information

Glycolytic profile shift and antioxidant triggering in symbiont-free and H2O2-resistant Strigomonas culicis

ABSTRACT: During their life cycle, trypanosomatids are exposed to stress conditions and adapt their energy and antioxidant metabolism to colonize their hosts. Strigomonas culicis is a monoxenous protist found in invertebrates with an endosymbiotic bacterium that completes essential biosynthetic pathways for the trypanosomatid. Our research group previously generated a wild-type H2O2-resistant (WTR) strain that showed improved mitochondrial metabolism and antioxidant defenses, which led to higher rates of Aedes aegypti infection. Here, we assess the biological contribution of the S. culicis endosymbiont and reactive oxygen species (ROS) resistance to oxidative and energy metabolism processes. Using high-throughput proteomics, several proteins involved in glycolysis and gluconeogenesis, the pentose phosphate pathway and glutathione metabolism were identified. The results suggest that ROS resistance decreases glucose consumption and indicate that the metabolic products from gluconeogenesis are key to supplying the protist with high-energy and reducing intermediates. Our hypothesis was confirmed by biochemical assays showing opposite profiles for glucose uptake and hexokinase and pyruvate kinase activity levels in the WTR and aposymbiotic strains, while the enzyme glucose-6P 1-dehydrogenase was more active in both strains. Regarding the antioxidant system, ascorbate peroxidase has an important role in H2O2 resistance and may be responsible for the high infection rates previously described for A. aegypti. In conclusion, our data indicate that the energy-related and antioxidant metabolic processes of S. culicis are modulated in response to oxidative stress conditions, providing new perspectives on the biology of the trypanosomatid-insect interaction as well as on the possible impact of resistant parasites in accidental human infection.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Strigomonas Culicis

TISSUE(S): Cell In Vitro, Cell Culture

DISEASE(S): Trypanosomiasis

SUBMITTER: Richard Hemmi Valente

LAB HEAD: Richard Hemmi Valente

PROVIDER: PXD015717 | Pride | 2019-11-27

REPOSITORIES: Pride

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Publications

Glycolytic profile shift and antioxidant triggering in symbiont-free and H2O2-resistant Strigomonas culicis.

Bombaça Ana Cristina Souza ACS Brunoro Giselle Villa Flor GVF Dias-Lopes Geovane G Ennes-Vidal Vitor V Carvalho Paulo Costa PC Perales Jonas J d'Avila-Levy Claudia Masini CM Valente Richard Hemmi RH Menna-Barreto Rubem Figueiredo Sadok RFS

Free radical biology & medicine 20191121

During their life cycle, trypanosomatids are exposed to stress conditions and adapt their energy and antioxidant metabolism to colonize their hosts. Strigomonas culicis is a monoxenous protist found in invertebrates with an endosymbiotic bacterium that completes essential biosynthetic pathways for the trypanosomatid. Our research group previously generated a wild-type H2O2-resistant (WTR) strain that showed improved mitochondrial metabolism and antioxidant defenses, which l ...[more]

PMID: 31760093

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Dataset Information

Glycolytic profile shift and antioxidant triggering in symbiont-free and H2O2-resistant Strigomonas culicis

Publications

Glycolytic profile shift and antioxidant triggering in symbiont-free and H<sub>2</sub>O<sub>2</sub>-resistant Strigomonas culicis.

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