Proteomics

Dataset Information

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Glycolytic profile shift and antioxidant triggering in symbiont-free and H2O2-resistant Strigomonas culicis


ABSTRACT: During their life cycle, trypanosomatids are exposed to stress conditions and adapt their energy and antioxidant metabolism to colonize their hosts. Strigomonas culicis is a monoxenous protist found in invertebrates with an endosymbiotic bacterium that completes essential biosynthetic pathways for the trypanosomatid. Our research group previously generated a wild-type H2O2-resistant (WTR) strain that showed improved mitochondrial metabolism and antioxidant defenses, which led to higher rates of Aedes aegypti infection. Here, we assess the biological contribution of the S. culicis endosymbiont and reactive oxygen species (ROS) resistance to oxidative and energy metabolism processes. Using high-throughput proteomics, several proteins involved in glycolysis and gluconeogenesis, the pentose phosphate pathway and glutathione metabolism were identified. The results suggest that ROS resistance decreases glucose consumption and indicate that the metabolic products from gluconeogenesis are key to supplying the protist with high-energy and reducing intermediates. Our hypothesis was confirmed by biochemical assays showing opposite profiles for glucose uptake and hexokinase and pyruvate kinase activity levels in the WTR and aposymbiotic strains, while the enzyme glucose-6P 1-dehydrogenase was more active in both strains. Regarding the antioxidant system, ascorbate peroxidase has an important role in H2O2 resistance and may be responsible for the high infection rates previously described for A. aegypti. In conclusion, our data indicate that the energy-related and antioxidant metabolic processes of S. culicis are modulated in response to oxidative stress conditions, providing new perspectives on the biology of the trypanosomatid-insect interaction as well as on the possible impact of resistant parasites in accidental human infection.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Strigomonas Culicis

TISSUE(S): Cell In Vitro, Cell Culture

DISEASE(S): Trypanosomiasis

SUBMITTER: Richard Hemmi Valente  

LAB HEAD: Richard Hemmi Valente

PROVIDER: PXD015717 | Pride | 2019-11-27

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20171012_C01_02ug_01.RAW Raw
20171012_C01_02ug_01.sqt Other
20171012_C01_02ug_02.RAW Raw
20171012_C01_02ug_02.sqt Other
20171012_C01_02ug_03.RAW Raw
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Publications

Glycolytic profile shift and antioxidant triggering in symbiont-free and H<sub>2</sub>O<sub>2</sub>-resistant Strigomonas culicis.

Bombaça Ana Cristina Souza ACS   Brunoro Giselle Villa Flor GVF   Dias-Lopes Geovane G   Ennes-Vidal Vitor V   Carvalho Paulo Costa PC   Perales Jonas J   d'Avila-Levy Claudia Masini CM   Valente Richard Hemmi RH   Menna-Barreto Rubem Figueiredo Sadok RFS  

Free radical biology & medicine 20191121


During their life cycle, trypanosomatids are exposed to stress conditions and adapt their energy and antioxidant metabolism to colonize their hosts. Strigomonas culicis is a monoxenous protist found in invertebrates with an endosymbiotic bacterium that completes essential biosynthetic pathways for the trypanosomatid. Our research group previously generated a wild-type H<sub>2</sub>O<sub>2</sub>-resistant (WTR) strain that showed improved mitochondrial metabolism and antioxidant defenses, which l  ...[more]

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