Proteomics

Dataset Information

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Identification of Stress Granule interactor proteins in yeast S. cerevisiae


ABSTRACT: We investigated the sub-cellular architecture of Stress Granules in the context of glucose starvation. In order to uncover transient and peripheral Stress Granule interactors, we optimized the proximity-dependent biotinylation (BioID) approach for use in yeast. This approach allowed us to visualize Stress Granule formation while simultaneously inducing the biotinylation of SG components and nearby proteins in live cells. We used two independent Stress Granule components Pub1 and Pab1.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Saccharomyces cerevisiae  

TISSUE(S): Tissue Not Applicable To Dataset

DISEASE(S): Not Available

SUBMITTER: Triana Amen  

LAB HEAD: Daniel Kaganovich

PROVIDER: PXD016988 | Pride | 2020-03-23

REPOSITORIES: pride

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Publications

Stress granules sense metabolic stress at the plasma membrane and potentiate recovery by storing active Pkc1.

Amen Triana T   Kaganovich Daniel D  

Science signaling 20200317 623


As the physical barrier between the cell and the outside environment, the plasma membrane is well-positioned to be the first responder to stress. The membrane is also highly vulnerable to many types of perturbation, including heat, force, osmotic pressure, lipid shortage, and starvation. To determine whether the structural changes in the plasma membrane of Saccharomyces cerevisiae brought about by nutrient stress can be communicated to regulatory networks within the cell, we identified proteins  ...[more]

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