Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Serum proteome profiling in canine chronic valve disease using TMT-based quantitative proteomics

ABSTRACT: Cardiovascular-related disorders (CVD) account for the highest mortality rates in the world, and affect the quality and the quantity of life of patients, creating an economic burden of prolonged therapeutic intervention. The aim of this study was to evaluate the serum protein profile of healthy and CVD affected dogs, by means of an isobaric tandem mass tag (TMT) label-based high-resolution quantitative proteomic approach.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Canis Familiaris (dog) (canis Lupus Familiaris)

TISSUE(S): Blood Serum

DISEASE(S): Cardiovascular System Disease

SUBMITTER: Anita Horvatic

LAB HEAD: Peter David Eckersall

PROVIDER: PXD017129 | Pride | 2020-05-26

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Similar Datasets

Canine pyometra - plasma proteomics

Project description:Canine pyometra is a common inflammatory disease of the uterus in sexually mature bitches, caused by a secondary bacterial infection which leads to change in the plasma protein associated with the innate immune system. Proteomic offers a means to determine the profile of plasma in dogs with pyometra to provide important findings into general mechanisms operating during diverse inflammatory reactions. The plasma protein profile of healthy and pyometra affected bitches was determined by means of an isobaric tandem mass tag (TMT) label-based high-resolution quantitative proteomic approach. Six clinically healthy dogs (used as control group) and 6 dogs with pyometra were enrolled in the study. All dogs were admitted to the Small Animal Clinic, University of Veterinary Medicine Hannover and treated in accordance with the German Animal Welfare Law. The experimental design was approved by the Animal Welfare officer of the University of Veterinary Medicine and by the Ethic Committee of the responsible authority (Lower Saxony State Office for Consumer Protection and Food Safety, reference number 17A 101). Healthy dogs for blood collection were recruited by launching a call in the University of Veterinary Medicine Hannover network for students and staff members for haemostasis study, providing a free clinical and laboratory health check of their animal in combination with the blood collection. Residual sample material was used in the present study.

2020-05-18 | PXD015951 | Pride

Serum proteomics in cats with cardiomyopathy and congestive heart failure

Project description:In this study, we aimed to identify novel serum biomarkers in feline primary cardiomyopathies with congestive heart failure, which can help to understand disease pathogenesis and assist the disease diagnosis, prognosis and management. The study included 15 cats diagnosed with CHF and CM, 5 cats with asymptomatic CM and 15 healthy cats.

2020-07-19 | PXD017761 | Pride

TMT-based proteomic analysis of CSF in dogs with intervertebral disc herniation

Project description:In our study, MS-based high-resolution proteomic analysis of CSF have been employed in order to gain deeper insights into changes occurring during IVDH in dogs, monitoring the pathophysiological processes at the protein and metabolite levels. The proteomics study involved canine patients classified within two groups. Healthy dogs (control group) (N=6) were mix breeds (20 kg average body weight), age 2-6 years, while the intervertebral disc herniation group (N=9) were mixed breeds (body weight 6-15 kg), age 7-12 years. In IVDH dogs, disc herniation was detected in T13/L1/L2 discs by CT. The dogs in IVDH group were paraplegic with preserved deep pain reflex and underwent a surgery procedure for decompression of the spinal cord.

2021-11-25 | PXD024921 | Pride

Proteomic investigation of treatment with medicinal mushroom supplement Agarikon.1® in advanced colorectal cancer animal model

Project description:Colorectal cancer (CRC) is the third most frequent cancer type in both males and females, with about 35% of patients being diagnosed in stage IV metastatic disease. Despite advancements in treatment, life expectancy in patients with metastatic disease is low. Due to frequent drug resistance during conventional and targeted cancer treatments, the development and testing of multi-target therapies is an important objective. Medicinal mushrooms specific compound isolates as well as complex extract mixtures have been studied in depth, and many mushroom species have been proven to be non-toxic multi-target inhibitors of specific oncogenic pathways, as well as potent immunomodulators. In this study, we have performed a tandem mass tags qualitative and quantitative proteomic analyses of CT26.WT colon cancer tumor tissues from Balb/c mice treated with proprietary medicinal mushroom extract Agarikon.1®, with or without 5-fluorouracil.

2020-10-27 | PXD018827 | Pride

Distinguishing natural infections of the bovine mammary gland by Staphylococcus from Streptococcus spp using quantitative milk proteomics

Project description:This study was aimed at determining the quantitative variations in the milk proteome caused by natural infection by Staphylococcus and Streptococcus species, in order to gain further understanding of any discrepancies in pathophysiology and host immune responses, independent of the mastitis level.

2023-06-29 | PXD041574 | Pride

TMT-based proteomics of longissimus lumborum muscle in newly weaned piglets after dietary Spirulina inclusion and enzyme supplementation

Project description:The objective of this study was to evaluate the impact of dietary Spirulina and lysozyme supplementation over the muscle proteome of piglets during the post-weaning stage. Thirty piglets were randomly distributed among three diets: control (no microalga), SP (10% Spirulina) and SP+L (10% Spirulina + 0.01% lysozyme). They were fed ad libitum for 4 weeks, after which they were sacrificed and samples of the longissimus lumborum muscle were taken. The muscle proteome was analysed using a Tandem Mass Tag (TMT)-based quantitative approach.

2021-05-24 | PXD024083 | Pride

Serum proteome profiling in canine babesiosis caused by Babesia rossi

Project description:The aim of this study was to compare the serum proteomes in dogs with uncomplicated and complicated babesiosis caused by B. rossi using a label-based high-throughput proteomics approach.

2023-06-27 | PXD039022 | Pride

Biomarkers for diagnosing the retained placenta in cows-does the analysis of serum proteome keep the secret?

Project description:Retained placenta (RP), a quite common disorder in dairy cows, shows a high negative impact on their health status and milk production. Therefore, the biomarkers indicating the risk for RP could yield benefits in dairy management. The study aimed to investigate the serum proteome as the source of the biomarkers for diagnosing the RP. Using the high-resolution liquid chromatography-tandem mass spectrometry, we compared the composition of serum proteome between 9 cows with the RP and 6 with the physiologic puerperium (PP). Among 651 identified proteins, seven had higher, and three showed lower abundance in RP than in the PP group. The differently abundant proteins participated in 15 pathways: six related to hemostasis, three involved in the lipoprotein metabolism, and the remaining ones associated with redox homeostasis, post-translational modification, scavenging, etc. Validation of the proteomic results, using ELISA and spectrophotometric assays, showed that the two identified proteins, haptoglobin, and lipopolysaccharide-binding protein, could represent reliable RP biomarkers. Our results confirmed that the analysis of serum proteome shed "the light" on the counterparty piece(s) of the RP pathogenesis "puzzle" and could be an efficient "tool" in mining for RP biomarkers.

2023-01-13 | PXD034963 | Pride

Changes in saliva associated to COVID-19: a proteomic study

Project description:The objective of this report was to perform a proteomic study of the saliva of SARS-CoV-2 patients.

2022-07-11 | PXD031318 | Pride

Revealing the changes in saliva and serum proteins of pigs with meningitis caused by Streptococcus suis : A complementary proteomic approach

Project description:The objective of this study was to assess the changes in the salivary proteome profile of piglets with meningitis and compare them with the serum proteome. The LC-MS/MS TMT proteomic approach was used to analyze saliva and serum samples from twenty male piglets: ten with meningitis and ten healthy.

2022-12-13 | PXD037430 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data