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A systematic, label-free method for identifying RNA-associated proteins in vivo provides insights into vertebrate ciliary beating - RNA DIF-FRAC Xenopus laevis Animal Cap explants Replicate 1

ABSTRACT: The associated files are mass spec data from size exclusion chromatographic separations of Xenopus laevis animal cap explants with and without RNAse A treatment.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Xenopus Laevis (african Clawed Frog)

TISSUE(S): Animal Cap, Early Embryonic Cell

SUBMITTER: Kevin Drew

LAB HEAD: Edward Marcotte

PROVIDER: PXD017659 | Pride | 2020-03-10

REPOSITORIES: Pride

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A systematic, label-free method for identifying RNA-associated proteins in vivo provides insights into vertebrate ciliary beating machinery.

Drew Kevin K Lee Chanjae C Cox Rachael M RM Dang Vy V Devitt Caitlin C CC McWhite Claire D CD Papoulas Ophelia O Huizar Ryan L RL Marcotte Edward M EM Wallingford John B JB

Developmental biology 20200906 1-2

Cell-type specific RNA-associated proteins are essential for development and homeostasis in animals. Despite a massive recent effort to systematically identify RNA-associated proteins, we currently have few comprehensive rosters of cell-type specific RNA-associated proteins in vertebrate tissues. Here, we demonstrate the feasibility of determining the RNA-associated proteome of a defined vertebrate embryonic tissue using DIF-FRAC, a systematic and universal (i.e., label-free) method. Application ...[more]

PMID: 32898505

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