Proteomics

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The proteome of Rickettsia akari revealed a 44kDa-OMP as novel biomarker for Rickettsialpox


ABSTRACT: Our study aimed to investigate the proteome structure of R. akari and unveil immunoreactive surface-exposed proteins using the latest proteomics techniques. To achieve these goals, we applied the Shotgun protein identification approach; membrane surface protein enrichment methods combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) and immunoblotting techniques. We identified aroun 300 unique proteins from the whole-cell extract exhibiting that the most represented proteins were those involved in translation, post-translational modifications, energy production, and cell wall development. A significant part belongs to amino acid transport and intracellular trafficking. Besides, several proteins affecting virulence were detected. Then, in silico analysis from detected surface proteins revealed that 25 putative predicted outer membrane proteins contain beta-barrel structure and 11 proteins were predicted to have secretion signal peptide sequence.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Rickettsia Akari

TISSUE(S): Bacterial Protein, Vero Cell, Egg

DISEASE(S): Rickettsialpox

SUBMITTER: Jiri DRESLER  

LAB HEAD: Jiri Dresler

PROVIDER: PXD017762 | Pride | 2020-07-13

REPOSITORIES: Pride

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Proteomic analysis of Rickettsia akari proposes a 44 kDa-OMP as a potential biomarker for Rickettsialpox diagnosis.

Csicsay František F   Flores-Ramirez Gabriela G   Zuñiga-Navarrete Fernando F   Bartošová Mária M   Fučíková Alena A   Pajer Petr P   Dresler Jiří J   Škultéty Ľudovít Ľ   Quevedo-Diaz Marco M  

BMC microbiology 20200708 1


<h4>Background</h4>Rickettsialpox is a febrile illness caused by the mite-borne pathogen Rickettsia akari. Several cases of this disease are reported worldwide annually. Nevertheless, the relationship between the immunogenicity of R. akari and disease development is still poorly understood. Thus, misdiagnosis is frequent. Our study is aiming to identify immunogenic proteins that may improve disease recognition and enhance subsequent treatment. To achieve this goal, two proteomics methodologies w  ...[more]

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