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Cdh1 (Fzr1) Interactome in Neuroblastoma (N2A) cells

ABSTRACT: In this study, we wanted to explore the protein interactors of Cdh1, a subunit of the E3 Ubiquitin-Ligase Cdh1-APC. Our data show that in addition to interacting with other components of the Cdh1-APC complex, Cdh1 interacts with regulators of translation.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Victor Faundez

LAB HEAD: Arielle Valdez-Sinon

PROVIDER: PXD018875 | Pride | 2020-04-29

REPOSITORIES: Pride

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Cdh1-APC Regulates Protein Synthesis and Stress Granules in Neurons through an FMRP-Dependent Mechanism.

Valdez-Sinon Arielle N AN Lai Austin A Shi Liang L Lancaster Carly L CL Gokhale Avanti A Faundez Victor V Bassell Gary J GJ

iScience 20200501 5

Maintaining a balance between protein degradation and protein synthesis is necessary for neurodevelopment. Although the E3 ubiquitin ligase anaphase promoting complex and its regulatory subunit Cdh1 (Cdh1-APC) has been shown to regulate learning and memory, the underlying mechanisms are unclear. Here, we have identified a role of Cdh1-APC as a regulator of protein synthesis in neurons. Proteomic profiling revealed that Cdh1-APC interacts with known regulators of translation, including stress gra ...[more]

PMID: 32434143

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