Benchmarking of different methods for obtaining high quality secretomics data

ABSTRACT: The main drawback of secretome analyses are contaminating proteins derived from culture medium or proteins released from dying cells which are hard to distinguish from proteins actively secreted by cells. Therefore, several approaches have been applied allowing to remove contamination from secretome analysis. Here, we will compare these approaches and will evaluate their potential to determine proteins secreted by unconventional secretory pathways.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lung, Fibroblast Of Dermis, Cell Culture, Mesenchymal Cell

DISEASE(S): Lung Cancer

SUBMITTER: Gereon Poschmann  

LAB HEAD: Gereon Poschmann

PROVIDER: PXD018895 | Pride | 2020-10-19

REPOSITORIES: Pride