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TMT-proteomics of sorted MC38+ tumor cells with high-fat diet

ABSTRACT: Obesity is a major cancer risk factor, but the underlying molecular mechanisms are not always known. In this study, we look at proteome remodeling in cancer cells with obesity, comparing tumor cells sorted from mice fed high-fat versus control diet. We conducted 10-plex TMT-proteomics on GFP+ MC38 colorectal adenocarcinoma tumor cells, sorted from subcutaneously implanted tumors 12 days after implantation. This study reveals molecular pathways that change in cancer cells with obesity that promote tumor growth.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Permanent Cell Line Cell

DISEASE(S): Colorectal Cancer

SUBMITTER: Brandon Gassaway

LAB HEAD: Steven Gygi

PROVIDER: PXD019495 | Pride | 2020-12-16

REPOSITORIES: Pride

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Obesity Shapes Metabolism in the Tumor Microenvironment to Suppress Anti-Tumor Immunity.

Ringel Alison E AE Drijvers Jefte M JM Baker Gregory J GJ Catozzi Alessia A García-Cañaveras Juan C JC Gassaway Brandon M BM Miller Brian C BC Juneja Vikram R VR Nguyen Thao H TH Joshi Shakchhi S Yao Cong-Hui CH Yoon Haejin H Sage Peter T PT LaFleur Martin W MW Trombley Justin D JD Jacobson Connor A CA Maliga Zoltan Z Gygi Steven P SP Sorger Peter K PK Rabinowitz Joshua D JD Sharpe Arlene H AH Haigis Marcia C MC

Cell 20201209 7

Obesity is a major cancer risk factor, but how differences in systemic metabolism change the tumor microenvironment (TME) and impact anti-tumor immunity is not understood. Here, we demonstrate that high-fat diet (HFD)-induced obesity impairs CD8<sup>+</sup> T cell function in the murine TME, accelerating tumor growth. We generate a single-cell resolution atlas of cellular metabolism in the TME, detailing how it changes with diet-induced obesity. We find that tumor and CD8<sup>+</sup> T cells dis ...[more]

PMID: 33301708

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