Global profiling of myristoylation in Toxoplasma gondii

ABSTRACT: N-myristoylation is a ubiquitous class of protein lipidation across eukaryotes and N-myristoyl transferase (NMT) has been proposed as an attractive drug target in several pathogens. Here we describe the first global chemoproteomic analysis of protein myristoylation in Toxoplasma gondii. Through quantitative mass spectrometry coupled with validated chemoproteomic tools (cleavable capture reagents (Broncel et al., 2015, Speers and Cravatt, 2005) and NMT inhibitor (Schlott et al., 2019)) that allow for experimental validation, we confidently identified 65 myristoylated proteins. This dataset represents a large fraction of the parasite’s myristoylated proteome and a prerequisite to investigate this modification in Toxoplasma.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Toxoplasma Gondii Rh

TISSUE(S): Foreskin Fibroblast, Epithelial Cell Line

DISEASE(S): Toxoplasmosis

SUBMITTER: Malgorzata Broncel  

LAB HEAD: Moritz Treeck

PROVIDER: PXD019677 | Pride | 2020-07-06

REPOSITORIES: Pride