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Expression profiling of mouse embryonic stem cells by mass spectrometry

ABSTRACT: Embryonic stem cells from B6 and NOD backgrounds were derived freshly in the presence of 2i. After 3-5 passages on feeders, ES cells were cultured in 2i media without any feeders for several passages. In order to identify differentially expressed genes and proteins, we performed RNA-Seq and mass spectromety analysis respectively. Among the differentially expressed genes, we identified several important players in innate and adaptive immunity. Several of these genes had been linked to onset of type-1 diabetes. Proteomics analysis was able to quantitative differences in protein expression among the B6 and NOD ES cell lines.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Embryonic Stem Cell

SUBMITTER: Lars Plate

LAB HEAD: R. Luke Wiseman

PROVIDER: PXD020055 | Pride | 2020-08-31

REPOSITORIES: Pride

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Publications

Premature Activation of Immune Transcription Programs in Autoimmune-Predisposed Mouse Embryonic Stem Cells and Blastocysts.

Kirak Oktay O Ke Eugene E Yang Kevin Y KY Schwarz Anna A Plate Lars L Nham Amy A Abadejos Justin R JR Valencia Anna A Wiseman R Luke RL Lui Kathy O KO Ku Manching M

International journal of molecular sciences 20200811 16

Autoimmune diabetes is a complex multifactorial disease with genetic and environmental factors playing pivotal roles. While many genes associated with the risk of diabetes have been identified to date, the mechanisms by which external triggers contribute to the genetic predisposition remain unclear. Here, we derived embryonic stem (ES) cell lines from diabetes-prone non-obese diabetic (NOD) and healthy C57BL/6 (B6) mice. While overall pluripotency markers were indistinguishable between newly der ...[more]

PMID: 32796510

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