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Dentification of Cross-linking partners of BpA modified p37 and p97 by photo cross-linking MS

ABSTRACT: we wanted to see where and how the protein p37 interacts with its partner proteins p97, PPI,Sds22 and I3. To this end p37 derivatives with genetically encoded crosslinking amino acid (p-benzoyl-L-phenylalanine) were generated. After incubation with the partner proteins, photocross-linking and tryptic digest the resulting cross-linked peptides were analysed by mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Farnusch Kaschani

LAB HEAD: Farnusch Kaschani

PROVIDER: PXD021182 | Pride | 2020-10-19

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	ACE_0342-02_JB02.mzML	Mzml
	ACE_0342-02_JB02.raw	Raw
	ACE_0475_JB02.mzML	Mzml
	ACE_0475_JB02.raw	Raw
	ACE_0504_JB02.fasta	Fasta

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Identification of Cross-linking partners of BpA modified p97 by photo cross-linking MS

Project description:We wanted to see where and how the protein p97 interacts with its partner proteins p37, PPI,Sds22 and I3. To this end a p97 derivative with genetically encoded crosslinking amino acid (p-benzoyl-L-phenylalanine) was generated. After incubation with the partner proteins, photocross-linking and tryptic digest the resulting cross-linked peptides were analysed by mass spectrometry.

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In vivo cross-linking supports a head-to-tail mechanism for regulation of the plant plasma membrane P-type H+-ATPase

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