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Different protein cargo of extracellular vesicles from yeast or hyphal Candida albicans cells

ABSTRACT: Extracellular vesicles (EVs) are shed from cells of almost every type of cell or organism. These nano- to micrometer scaled lipid bilayer delimited spheres shuttle a set of bioactive molecules involved in many processes including cell-cell communication, host-pathogen interactions and even the sharing of microbial community resources. On the other hand, the yeast-to hypha transition is the most studied morphology transition in C. albicans and one of the most important traits of C. albicans to invade host tissue during infection. In this context, we have undergone the isolation and protein cargo characterization of EVs secreted by both major C. albicans morphologies by means, LC- MS analysis. A total of 2546 different proteins were identified from hyphal EVs in the 3 biological replicates carried out dropping this number to only 414 proteins identified in the 3 biological replicates from yeast EVs. Comparing the identified proteins of both EVs, 241 proteins were common to both type of vesicles. In terms of biological significance, we only took into consideration proteins that were identified in at least two biological replicates with at least two peptides and a q-value< 0.01.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Candida Albicans (yeast)

TISSUE(S): Extracellular Vesicle

SUBMITTER: Concha Gil

LAB HEAD: Concha Gil

PROVIDER: PXD021488 | Pride | 2022-04-07

REPOSITORIES: Pride

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