Proteomics

Dataset Information

A quantitative proteomics approach to monitor targeted protein degradation in M. smegmatis

ABSTRACT: Targeted protein degradation via the ubiquitin proteasome has been established in eukaryotes, however no application has been shown in bacteria so far. In the present work, selective protein degradation is shown using hetero bifunctional drugs, bringing substrates in close proximity to the bacterial ClpC1P1P2 protein degradation complex. We performed a TMT based quantitative proteomics analysis to monitor degradation of human BRDT in Mycobacterium smegmatis. Our data shows selective degradation of BRDT when using bacterial PROTACS, indicating the first successful application of targeted protein degradation in bacteria.

INSTRUMENT(S):

ORGANISM(S): Mycobacterium Smegmatis (strain Atcc 700084 / Mc(2)155) Bacteria

SUBMITTER: David Hoi  

LAB HEAD: Markus Hartl

PROVIDER: PXD021505 | Pride | 2022-08-12

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
2019.05_UP000000757_Mycobacterium_smegmatis_all_BRDT.fasta Fasta
20200424_QExHFX1_RSLC1_Hoi_Clausen_IMP_Msm_tmt_rep1_frac.msf Msf
20200424_QExHFX1_RSLC1_Hoi_Clausen_IMP_Msm_tmt_rep1_frac1.raw Raw
20200424_QExHFX1_RSLC1_Hoi_Clausen_IMP_Msm_tmt_rep1_frac10.raw Raw
20200424_QExHFX1_RSLC1_Hoi_Clausen_IMP_Msm_tmt_rep1_frac2.raw Raw
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