Structural analysis of neurofibromin by crosslinking mass spectrometry

ABSTRACT: Neurofibromin (NF1) is a fundamental inhibitor of cell growth that is conserved from yeast to humans. NF1 negatively regulates oncogenic RAS proteins by accelerating the hydrolysis of RAS-bound GTP. This activity blocks growth factor signalling upstream of both mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) pathways. However, the structure and regulatory mechanisms of NF1 have remained elusive. Here we report crosslinking mass spectrometry analysis of the 320 kDa NF1 protein.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Ralf Schittenhelm  

LAB HEAD: Andrew Ellisdon

PROVIDER: PXD023593 | Pride | 2022-08-12

REPOSITORIES: Pride