Optimized Sample Preparation Workflow for Improved Identification of Ghost Proteins

ABSTRACT: Large scale proteomic strategies rely on database interrogation. Thus, only referenced proteins can be identified. Recently, Alternative Proteins (AltProts) translated from nonannotated Alternative Open reading frame (AltORFs) were discovered using customized databases. Because of their small size which confers them peptide-like physicochemical properties, they are more difficult to detect using standard proteomics strategies. In this study, we tested different preparation workflows for improving the identification of AltProts in NCH82 human glioma cell line. The highest number of identified AltProts was achieved with RIPA buffer or boiling water extraction followed by acetic acid precipitation.

OTHER RELATED OMICS DATASETS IN: MODEL1109130000 MODEL1909260003 MODEL1909260004 MODEL1703310000 MODEL1909260005 MODEL1909260006 MODEL1311110000 MODEL1311110001

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

DISEASE(S): Brain Glioblastoma Multiforme

SUBMITTER: Tristan Cardon  

LAB HEAD: Michel Salzet

PROVIDER: PXD024134 | Pride | 2021-02-15

REPOSITORIES: Pride