Proteomics

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Interactome of Toxoplasma gondii Pre-mRNA 3’ Processing Complexes

ABSTRACT: A proper 3’end processing of mRNAs is regarded as one of the regulatory cornerstones of gene expression. In a parasite that must answer to the high regulatory requirements of its multi-host life style, the need is grand for adopting additional means to partition the distinct transcriptional signatures of the closely and tandemly-arranged stage specific genes. In this study, we report on our findings in T. gondii of an m6A-dependent 3’end polyadenylation serving as a transcriptional barrier at these loci. We identify the core polyadenylation complex within T. gondii and establish CPSF4 as a reader for m6A-modified mRNAs, via a YTH domain within its C-terminus, a feature which is shared with plants.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Homo Sapiens (human) Toxoplasma Gondii Rh

SUBMITTER: Yohann Couté  

LAB HEAD: Virginie Brun

PROVIDER: PXD024326 | Pride | 2021-07-16

REPOSITORIES: Pride

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Publications

A plant-like mechanism coupling m6A reading to polyadenylation safeguards transcriptome integrity and developmental gene partitioning in <i>Toxoplasma</i>.

Farhat Dayana C DC   Bowler Matthew W MW   Communie Guillaume G   Pontier Dominique D   Belmudes Lucid L   Mas Caroline C   Corrao Charlotte C   Couté Yohann Y   Bougdour Alexandre A   Lagrange Thierry T   Hakimi Mohamed-Ali MA   Swale Christopher C  

eLife 20210715

Correct 3'end processing of mRNAs is one of the regulatory cornerstones of gene expression. In a parasite that must adapt to the regulatory requirements of its multi-host life style, there is a need to adopt additional means to partition the distinct transcriptional signatures of the closely and tandemly arranged stage-specific genes. In this study, we report our findings in <i>T. gondii</i> of an m6A-dependent 3'end polyadenylation serving as a transcriptional barrier at these <i>loci</i>. We i  ...[more]

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