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Metaproteomics of Human Breast Milk and Formula-fed Neonatal Piglets

ABSTRACT: The benefits of breastfeeding infants are well characterized, including those on the immune system. However, determining the mechanism by which human breast milk (HBM) elicits effects on immune response requires investigation in an appropriate animal model. In the current study we used neonatal piglets and compared their gut microbiome using mass spectrometry based metaproteomics

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Cecum

SUBMITTER: Boris Zybailov

LAB HEAD: Boris Zybailov

PROVIDER: PXD025432 | Pride | 2021-11-03

REPOSITORIES: Pride

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Milk Formula Diet Alters Bacterial and Host Protein Profile in Comparison to Human Milk Diet in Neonatal Piglet Model.

Rosa Fernanda F Zybailov Boris L BL Glazko Galina V GV Rahmatallah Yasir Y Byrum Stephanie S Mackintosh Samuel G SG Bowlin Anne K AK Yeruva Laxmi L

Nutrients 20211022 11

The metaproteome profiling of cecal contents collected from neonatal piglets fed pasteurized human milk (HM) or a dairy-based infant formula (MF) from postnatal day (PND) 2 to 21 were assessed. At PND 21, a subset of piglets from each group (<i>n</i> = 11/group) were euthanized, and cecal contents were collected for further metaproteome analysis. Cecal microbiota composition showed predominantly more Firmicutes phyla and <i>Lachnospiraceae</i> family in the lumen of cecum of HM-fed piglets in co ...[more]

PMID: 34835974

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