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Systematic analysis of the ubiquitome in mouse testis

ABSTRACT: The ubiquitome was systematically investigated in mouse testis using di-glycine remnant antibodies by LC-MS/MS.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Testis

SUBMITTER: yao ping

LAB HEAD: Xuejiang Guo

PROVIDER: PXD025866 | Pride | 2021-06-03

REPOSITORIES: Pride

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	mmtestis-ubiquitylation-15mg-20130925.zip	Other
	mmtestis-ubiquitylation-15mg-fraction1-20130925.raw	Raw
	mmtestis-ubiquitylation-15mg-fraction1-20131101.raw	Raw
	mmtestis-ubiquitylation-15mg-fraction10-20130930.raw	Raw
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Systematic analysis of the ubiquitome in mouse testis.

Guo Yueshuai Y Zhang Haotian H Yao Liping L Li Yan Y Situ Chenghao C Sha Jiahao J Chen Daozhen D Guo Xuejiang X

Proteomics 20210606 15

A growing body of evidence now supports the fact that protein ubiquitination is an important modification during the regulation of spermatogenesis. However, little is known about the ubiquitome of the testis. In this study, we created a large-scale mouse testis ubiquitome profile using di-glycine remnant antibodies and mass spectrometry and identified a total of 14,219 ubiquitination sites in 4217 proteins. Bioinformatics and phenotypic analyses showed that the ubiquitinated proteins were closel ...[more]

PMID: 34050602

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