ABSTRACT: Prevotella bryantii B14 was cultivated with monensin. Growth was monitored over a period of 9h with a broad range of monensin concentrations.
Project description:Prevotella bryantii B14 was cultivated with monensin. Growth was monitored over a period of 72h including frequent sampling of cells.
Project description:The time-resolved impact of monensin on the active rumen microbiome in a rumen-simulating technique (Rusitec) was studied with metaproteomic and metabolomic approaches. Upon monensin treatment, decreased catabolism linked to fiber degradation was observed by the reduced abundance of proteins assigned to fibrolytic bacteria and glycoside hydrolases, sugar transporters and carbohydrate metabolism. Reduced amounts of ammonium as well as branched-chain fatty acids pointed towards a decreased proteolytic activity. The family Prevotellaceae exhibited increased resilience in the presence of monensin, with a switch of metabolism from acetate to succinate production. Prevotella species harbor a membrane bound electron transfer complex, which drives the reduction of fumarate to succinate, the substrate for propionate production in the rumen habitat. Besides the increased succinate production, a concomitant depletion of methane concentration was observed upon monensin exposure. Our study demonstrates that Prevotella sp. shifts its metabolism successfully in response to monensin exposure and Prevotellaceae represents the key bacterial family stabilizing the rumen microbiota during exposure to monensin.
Project description:The aim of the present work was to investigate the effect of monensin on the in vitro growth of T. gondii tachyzoites and on the host cells (human brain microvascular endothelial cells - hBMECs). The hypotheses were that (1) inhibition of the WNT signalling pathway by monensin can reduce the growth of T. gondii infecting human brain microvascular endothelial cells (hBMECs) and (2) by suppression of the growth of T. gondii using monensin, impairment of the BBB integrity can be restored (3) inhibition of WNT pathway by monensin can be detected by microarray experiment.
Project description:P. bryantii B14 cells were cultivated separately in acetic (Acet), propionic (Prop), butyric (But), iso-butyric (iBut), valeric (Val), iso-valeric (iVal) and 2-methyl butyric acid (2MB) as well as in a mixture of all mentioned short-chain fatty acids (Mix). All 8 treatments were analyzed regarding their proteomes in order to understand the requirements and effects of each SCFA on the metabolism.
Project description:A549 cells were treated for 8 or 20 h using 20 ng/mL brefeldin A (BFA), 5 µM golgicide A (GCA), 10 µM monensin (MON) or the appropriate vehicle control (BFA/EtOH, MON/EtOH, GCA/DMSO).
Project description:This project aimed to identify key proteins dysregulated by monensin and itssynthetic analog, compound 12, in the triple-negative breast cancer (TNBC) cell line MDA-MB-231. Byanalyzing the molecular changes induced by these compounds, the study sought to elucidate themechanisms underlying their cytotoxic effects on breast cancer cells. Additionally, it explored potentialdifferences in the activity and efficacy of monensin and compound 12, providing critical insights into theirdistinct biological impacts. These findings may contribute to the development of targeted therapeuticstrategies for triple-negative breast cancer, a highly aggressive and treatment-resistant subtype of breastcancer
Project description:Mismanagement of plastic waste has contributed to plastic pollution in marine and terrestrial ecosystems. Therefore, farm animals are likely to consume microplastic (MP) contaminated feed. However, the interactions of MP with the ruminal microbial ecosystem remain poorly understood. This study investigated the interaction of MP within the ruminal ecosystem in vitro using the Hohenheim Gas Test. Different MP variants were applied, reflecting different combinations of five MP species (polylactide, polyhydroxy butyric acid, high-density polyethylene, polyvinyl chloride, polypropylene), in two particle size ranges (<125 μm; 125-500 μm) and increasing dosages (from 0 to 70 mg /incubation cylinder) together with ruminal fluid and hay or barley as substrates. Cumulative gas production, pH and dry matter disappearance were determined before analyzing volatile fatty acids, metaproteomics and metabolomics. In the presence of MP, cumulative gas production decreased regardless of the MP 63 species, dose or particle size, while total dry matter degradation increased. Microbial proteins in barley incubations showed lower Bacteroidetes and increasing Firmicutes abundance in the presence of MP and increased activities of the protein groups `replication and repair`, and `translation`, but decreased activities of `carbohydrate metabolism and transport` and `amino acids metabolism`. The data indicated that MP, regardless of their species and particle size, interact with the ruminal microbiome and may be partially degraded in vitro. This suggests a reduction of MP size in the rumen thereby increasing the likelihood to penetrate animal tissues. Future research must confirm the findings in vivo and determine their consequences for animal health and consumer safety.
Project description:In order to better understand how the coccidiocide monensin affects T. gondii, we analyzed the effect of monensin on gene expression of intracellular parasites through microarrays.
Project description:In order to better understand how the coccidiocide monensin affects T. gondii, we analyzed the effect of monensin on gene expression of intracellular parasites through microarrays. We compared RNA collected either from RH strain tachyzoites 24 hrs after invasion of human foreskin fibroblasts, or 24 hrs after invasion followed by 24 hrs exposure to 2.5ng monensin/ml in tissue culture medium.