Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Prevotella_monensin_time series

ABSTRACT: Prevotella bryantii B14 was cultivated with monensin. Growth was monitored over a period of 72h including frequent sampling of cells.

INSTRUMENT(S):

ORGANISM(S): Prevotella Bryantii B14

SUBMITTER: Jana Seifert

LAB HEAD: Prof. Jana Seifert

PROVIDER: PXD026057 | Pride | 2021-10-13

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	24h_0_A.raw	Raw
	24h_0_B.raw	Raw
	24h_0_C.raw	Raw
	24h_20_A.raw	Raw
	24h_20_B.raw	Raw

Items per page:

1 - 5 of 35

Similar Datasets

Prevotella monensin concentration

Project description:Prevotella bryantii B14 was cultivated with monensin. Growth was monitored over a period of 9h with a broad range of monensin concentrations.

2021-10-13 | PXD026085 | Pride

Cytotoxic and anti-Toxoplasma gondii activities of monensin on human brain microvascular endothelial cells

Project description:The aim of the present work was to investigate the effect of monensin on the in vitro growth of T. gondii tachyzoites and on the host cells (human brain microvascular endothelial cells - hBMECs). The hypotheses were that (1) inhibition of the WNT signalling pathway by monensin can reduce the growth of T. gondii infecting human brain microvascular endothelial cells (hBMECs) and (2) by suppression of the growth of T. gondii using monensin, impairment of the BBB integrity can be restored (3) inhibition of WNT pathway by monensin can be detected by microarray experiment.

2020-07-06 | E-MTAB-8817 | biostudies-arrayexpress

Rumen microbiome and effect of monensin

Project description:The time-resolved impact of monensin on the active rumen microbiome in a rumen-simulating technique (Rusitec) was studied with metaproteomic and metabolomic approaches. Upon monensin treatment, decreased catabolism linked to fiber degradation was observed by the reduced abundance of proteins assigned to fibrolytic bacteria and glycoside hydrolases, sugar transporters and carbohydrate metabolism. Reduced amounts of ammonium as well as branched-chain fatty acids pointed towards a decreased proteolytic activity. The family Prevotellaceae exhibited increased resilience in the presence of monensin, with a switch of metabolism from acetate to succinate production. Prevotella species harbor a membrane bound electron transfer complex, which drives the reduction of fumarate to succinate, the substrate for propionate production in the rumen habitat. Besides the increased succinate production, a concomitant depletion of methane concentration was observed upon monensin exposure. Our study demonstrates that Prevotella sp. shifts its metabolism successfully in response to monensin exposure and Prevotellaceae represents the key bacterial family stabilizing the rumen microbiota during exposure to monensin.

2022-12-11 | PXD034099 | Pride

Prevotella bryantii B14 proteins from in vitro cultivation under various short-chain fatty acids (SCFA)

Project description:P. bryantii B14 cells were cultivated separately in acetic (Acet), propionic (Prop), butyric (But), iso-butyric (iBut), valeric (Val), iso-valeric (iVal) and 2-methyl butyric acid (2MB) as well as in a mixture of all mentioned short-chain fatty acids (Mix). All 8 treatments were analyzed regarding their proteomes in order to understand the requirements and effects of each SCFA on the metabolism.

2021-09-09 | PXD016407 | Pride

Transcription profiling by array of human prostate cancer cells treated with monensin to investigate the effect on apoptosis induction and oxidative stress

Project description:Effect of monensin on apoptosis induction and oxidative stress in prostate cancer cells.

2011-07-30 | E-TABM-1049 | biostudies-arrayexpress

Microarray analysis of human A549 lung cancer cells treated with brefeldin A, golgicide A, monensin or vehicle control for 8 h or 20 h

Project description:A549 cells were treated for 8 or 20 h using 20 ng/mL brefeldin A (BFA), 5 ÂµM golgicide A (GCA), 10 ÂµM monensin (MON) or the appropriate vehicle control (BFA/EtOH, MON/EtOH, GCA/DMSO).

2017-12-18 | E-MTAB-5627 | biostudies-arrayexpress

Evaluation of the activity of monensin and its analogs in triple negative breast cancer cells

Project description:This project aimed to identify key proteins dysregulated by monensin and itssynthetic analog, compound 12, in the triple-negative breast cancer (TNBC) cell line MDA-MB-231. Byanalyzing the molecular changes induced by these compounds, the study sought to elucidate themechanisms underlying their cytotoxic effects on breast cancer cells. Additionally, it explored potentialdifferences in the activity and efficacy of monensin and compound 12, providing critical insights into theirdistinct biological impacts. These findings may contribute to the development of targeted therapeuticstrategies for triple-negative breast cancer, a highly aggressive and treatment-resistant subtype of breastcancer

2026-03-30 | PXD060697 | Pride

Toxoplasma gondii response to monensin

2010-11-06 | GSE25177 | GEO

Toxoplasma gondii response to monensin

Project description:In order to better understand how the coccidiocide monensin affects T. gondii, we analyzed the effect of monensin on gene expression of intracellular parasites through microarrays. We compared RNA collected either from RH strain tachyzoites 24 hrs after invasion of human foreskin fibroblasts, or 24 hrs after invasion followed by 24 hrs exposure to 2.5ng monensin/ml in tissue culture medium.

2010-11-06 | E-GEOD-25177 | biostudies-arrayexpress

Expression profiling by high throughput sequencing of THP-1 cells treated with Monensin

Project description:Purpose: to reveal gene expression changes induced by Monensin in THP-1 cells

2020-04-28 | GSE130657 | GEO