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Mouse Heart SS-31 Proteome and Phosphoproteome

ABSTRACT: The heart proteome and phosphoproteome were analyzed in young (5-6-month old), old (24-month old at the start of the study), and elamipretide-treated (for 8 weeks) old mice using shotgun proteomics methods in order to assess the effects of aging on signaling and the ability of mitochondrion-targeted drug elamipretide (SS-31) to reverse age-related changes. Enhancement and suppression of phosphorylation at sites along a variety of proteins was found to occur with age. Elamipretide treatment partially restored the phosphorylation state of proteins that had increased phosphorylation with age, but did not have a large effect those that had decreased phosphorylation with age.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Heart

SUBMITTER: Judit Villen

LAB HEAD: Judit Villen

PROVIDER: PXD026335 | Pride | 2022-02-17

REPOSITORIES: Pride

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Elamipretide (SS-31) treatment attenuates age-associated post-translational modifications of heart proteins.

Whitson Jeremy A JA Martín-Pérez Miguel M Zhang Tong T Gaffrey Matthew J MJ Merrihew Gennifer E GE Huang Eric E White Collin C CC Kavanagh Terrance J TJ Qian Wei-Jun WJ Campbell Matthew D MD MacCoss Michael J MJ Marcinek David J DJ Villén Judit J Rabinovitch Peter S PS

GeroScience 20210904 5

It has been demonstrated that elamipretide (SS-31) rescues age-related functional deficits in the heart but the full set of mechanisms behind this have yet to be determined. We investigated the hypothesis that elamipretide influences post-translational modifications to heart proteins. The S-glutathionylation and phosphorylation proteomes of mouse hearts were analyzed using shotgun proteomics to assess the effects of aging on these post-translational modifications and the ability of the mitochond ...[more]

PMID: 34480713

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