Proteomics

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Gendered burial practices of early Bronze Age children align with peptide-based sex identification: a case study from Franzhausen I, Austria


ABSTRACT: Gendered burial practices that differentiate between men and women by the way the body was placed were used over large parts of Central Europe in the Late Neolithic and Early Bronze Age (c. 2900−1600 BC). The differentiation of bodies placed on the left/right side in opposite orientation was extended to children, but until recently, it was difficult to confirm if the biological sex of the children matched the classification as men and women. We applied nanoflow liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) to identify sex-specific peptides in human tooth enamel in 75 children buried at one of the largest Early Bronze Age cemeteries in Europe, Franzhausen I, Austria, 70 of which produced reliable results. The study confirmed that the sex of the children corresponds to the gendered body position in 98.4 % of cases. For burials in which the gendered sidedness and orientation are not internally consistent with the male or female pattern, we found that the sidedness of the body corresponds to the sex of the children rather than the orientation.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Christopher Gerner  

LAB HEAD: Christopher Gerner

PROVIDER: PXD026419 | Pride | 2022-06-24

REPOSITORIES: Pride

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Publications

Child murder in the Early Bronze Age: proteomic sex identification of a cold case from Schleinbach, Austria.

Rebay-Salisbury Katharina K   Janker Lukas L   Pany-Kucera Doris D   Schuster Dina D   Spannagl-Steiner Michaela M   Waltenberger Lukas L   Salisbury Roderick B RB   Kanz Fabian F  

Archaeological and anthropological sciences 20201023 11


The identification of sex-specific peptides in human tooth enamel by nanoflow liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) represents a quantum leap for the study of childhood and social relations more generally. Determining sex-related differences in prehistoric child rearing and mortality has been hampered by the insufficient accuracy in determining the biological sex of juveniles. We conducted mass spectrometric analysis to identify sex-specific peptides in the dental enamel  ...[more]

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