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In-bone digestion as a way to characterization of human maxillary and mandibular bone tissues

ABSTRACT: The project is based on in-bone digestion of proteins by trypsin directly in human maxillary and mandibular healthy and pathological bone samples without the prior reduction and alkylation of the disulfide bonds. The released peptides were then separated and identified by LC-MS/MS method.

INSTRUMENT(S): maXis

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Alveolar Bone, Bone Of Jaw

SUBMITTER: Jiri Santrucek

LAB HEAD: Radovan Hynek

PROVIDER: PXD026775 | Pride | 2021-09-07

REPOSITORIES: Pride

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In-bone protein digestion followed by LC-MS/MS peptide analysis as a new way towards the routine proteomic characterization of human maxillary and mandibular bone tissue in oral surgery.

Hynek Radovan R Michalus Iva I Cejnar Pavel P Šantrůček Jiří J Seidlová Sabina S Kučková Štěpánka Š Sázelová Petra P Kašička Václav V

Electrophoresis 20210912 23

Proteomic characterization of alveolar bones in oral surgery represents an analytical challenge due to their insoluble character. The implementation of a straightforward technique could lead to the routine use of proteomics in this field. This work thus developed a simple technique for the characterization of bone tissue for human maxillary and mandibular bones. It is based on the direct in-bone tryptic digestion of proteins in both healthy and pathological human maxillary and mandibular bone sa ...[more]

PMID: 34453862

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